Piper sarmentosum attenuates TNF-α-induced VCAM-1 and ICAM-1 expression in human umbilical vein endothelial cells

Siti M. Ismail, Uma M. Sundar, Chua K. Hui, Amilia Aminuddin, Azizah Ugusman

Research output: Contribution to journalArticle

Abstract

Objectives: Inflammation plays a key role in the pathogenesis of atherosclerosis. Piper sarmentosum is an herb with antioxidant and anti-atherosclerotic activities. The aim of this study was to evaluate the anti-inflammatory properties of an aqueous extract of P sarmentosum (AEPS) in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were divided into six groups: control, treatment with 10 ng/ml TNF-α, and co-treatment of 10 ng/ml TNF-α with four different concentrations of AEPS (100, 150, 250, and 300 μg/ml) for 24 h. Subsequently, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) protein expression, U937 monocyte cells adhesion, and nuclear factor-kappaB (NF-κB) p65 expression in HUVECs were measured. Results: Treatment of TNF-α-stimulated HUVECs with AEPS at different concentrations resulted in decreased VCAM-1 and ICAM-1 protein expression in a dose-dependent manner. Furthermore, AEPS also inhibited TNF-α-stimulated U937 monocyte cells adhesion to HUVECs. In addition, AEPS reduced TNF-α-induced NF-κB p65 expression in a dose-dependent manner. Conclusions: The results indicated that AEPS suppressed TNF-α-induced VCAM-1 and ICAM-1 expression NF-κB signaling.

LanguageEnglish
JournalJournal of Taibah University Medical Sciences
DOIs
StateAccepted/In press - 1 Jan 2018

Fingerprint

Piper
Vascular Cell Adhesion Molecule-1
Human Umbilical Vein Endothelial Cells
Intercellular Adhesion Molecule-1
Tumor Necrosis Factor-alpha
Inflammation
U937 Cells
Monocytes
Cell Adhesion
Atherosclerosis
Proteins
Anti-Inflammatory Agents
Antioxidants
Control Groups

Keywords

  • Human umbilical vein endothelial cells
  • Inflammation
  • Intercellular adhesion molecule-1
  • Piper sarmentosum
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Piper sarmentosum attenuates TNF-α-induced VCAM-1 and ICAM-1 expression in human umbilical vein endothelial cells",
abstract = "Objectives: Inflammation plays a key role in the pathogenesis of atherosclerosis. Piper sarmentosum is an herb with antioxidant and anti-atherosclerotic activities. The aim of this study was to evaluate the anti-inflammatory properties of an aqueous extract of P sarmentosum (AEPS) in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were divided into six groups: control, treatment with 10 ng/ml TNF-α, and co-treatment of 10 ng/ml TNF-α with four different concentrations of AEPS (100, 150, 250, and 300 μg/ml) for 24 h. Subsequently, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) protein expression, U937 monocyte cells adhesion, and nuclear factor-kappaB (NF-κB) p65 expression in HUVECs were measured. Results: Treatment of TNF-α-stimulated HUVECs with AEPS at different concentrations resulted in decreased VCAM-1 and ICAM-1 protein expression in a dose-dependent manner. Furthermore, AEPS also inhibited TNF-α-stimulated U937 monocyte cells adhesion to HUVECs. In addition, AEPS reduced TNF-α-induced NF-κB p65 expression in a dose-dependent manner. Conclusions: The results indicated that AEPS suppressed TNF-α-induced VCAM-1 and ICAM-1 expression NF-κB signaling.",
keywords = "Human umbilical vein endothelial cells, Inflammation, Intercellular adhesion molecule-1, Piper sarmentosum, Vascular cell adhesion molecule-1",
author = "Ismail, {Siti M.} and Sundar, {Uma M.} and Hui, {Chua K.} and Amilia Aminuddin and Azizah Ugusman",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.jtumed.2018.01.003",
language = "English",
journal = "Journal of Taibah University Medical Sciences",
issn = "1658-3612",
publisher = "Elsevier BV",

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TY - JOUR

T1 - Piper sarmentosum attenuates TNF-α-induced VCAM-1 and ICAM-1 expression in human umbilical vein endothelial cells

AU - Ismail,Siti M.

AU - Sundar,Uma M.

AU - Hui,Chua K.

AU - Aminuddin,Amilia

AU - Ugusman,Azizah

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Objectives: Inflammation plays a key role in the pathogenesis of atherosclerosis. Piper sarmentosum is an herb with antioxidant and anti-atherosclerotic activities. The aim of this study was to evaluate the anti-inflammatory properties of an aqueous extract of P sarmentosum (AEPS) in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were divided into six groups: control, treatment with 10 ng/ml TNF-α, and co-treatment of 10 ng/ml TNF-α with four different concentrations of AEPS (100, 150, 250, and 300 μg/ml) for 24 h. Subsequently, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) protein expression, U937 monocyte cells adhesion, and nuclear factor-kappaB (NF-κB) p65 expression in HUVECs were measured. Results: Treatment of TNF-α-stimulated HUVECs with AEPS at different concentrations resulted in decreased VCAM-1 and ICAM-1 protein expression in a dose-dependent manner. Furthermore, AEPS also inhibited TNF-α-stimulated U937 monocyte cells adhesion to HUVECs. In addition, AEPS reduced TNF-α-induced NF-κB p65 expression in a dose-dependent manner. Conclusions: The results indicated that AEPS suppressed TNF-α-induced VCAM-1 and ICAM-1 expression NF-κB signaling.

AB - Objectives: Inflammation plays a key role in the pathogenesis of atherosclerosis. Piper sarmentosum is an herb with antioxidant and anti-atherosclerotic activities. The aim of this study was to evaluate the anti-inflammatory properties of an aqueous extract of P sarmentosum (AEPS) in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were divided into six groups: control, treatment with 10 ng/ml TNF-α, and co-treatment of 10 ng/ml TNF-α with four different concentrations of AEPS (100, 150, 250, and 300 μg/ml) for 24 h. Subsequently, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) protein expression, U937 monocyte cells adhesion, and nuclear factor-kappaB (NF-κB) p65 expression in HUVECs were measured. Results: Treatment of TNF-α-stimulated HUVECs with AEPS at different concentrations resulted in decreased VCAM-1 and ICAM-1 protein expression in a dose-dependent manner. Furthermore, AEPS also inhibited TNF-α-stimulated U937 monocyte cells adhesion to HUVECs. In addition, AEPS reduced TNF-α-induced NF-κB p65 expression in a dose-dependent manner. Conclusions: The results indicated that AEPS suppressed TNF-α-induced VCAM-1 and ICAM-1 expression NF-κB signaling.

KW - Human umbilical vein endothelial cells

KW - Inflammation

KW - Intercellular adhesion molecule-1

KW - Piper sarmentosum

KW - Vascular cell adhesion molecule-1

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