Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide

Abdul Hamid Hafizah, Zaiton Zakaria, Amom Zulkhairi, Adenan Mohd Ilham, Megat Mohd Nordin Nor Anita, Zaleha Abdullah Mahdy

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Endothelial cell death due to increased reactive oxygen species (ROS) may contribute to the initial endothelial injury, which promotes atherosclerotic lesion formation. Piper sarmentosum (PS), a natural product, has been shown to have an antioxidant property, which is hypothesized to inhibit production of ROS and prevent cell injury. Thus, the present study was designed to determine the effects of PS on the hydrogen peroxide (H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells (HUVECs). In this experiment, HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation (LSGS). HUVECs were treated with various concentrations of H2O2 (01000 μmol/L) and it was observed that 180 μmol/L H2O2 reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Using the above concentration as the positive control, the H 2O2-induced HUVECs were concomitantly treated with various concentrations (100, 150, 250 and 300 μg/ml) of three different extracts (aqueous, methanol and hexane) of PS. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels showed a significant increase (P<0.05) in HUVECs compared to the negative control. However, PS extracts showed a protective effect on HUVECs from H 2O2-induced cell apoptosis with a significant reduction in MDA, SOD, CAT and GPX levels (P<0.05). Furthermore, PS had exhibited ferric reducing antioxidant power with its high phenolic content. Hence, it was concluded that PS plays a beneficial role in reducing oxidative stress in H 2O2-induced HUVECs.

Original languageEnglish
Pages (from-to)357-365
Number of pages9
JournalJournal of Zhejiang University: Science B
Volume11
Issue number5
DOIs
Publication statusPublished - May 2010

Fingerprint

Piper
Oxidative stress
Endothelial cells
Human Umbilical Vein Endothelial Cells
Hydrogen Peroxide
hydrogen peroxide
Oxidative Stress
oxidative stress
Antioxidants
antioxidants
Glutathione Peroxidase
Malondialdehyde
glutathione peroxidase
Catalase
malondialdehyde
Superoxide Dismutase
reactive oxygen species
Reactive Oxygen Species
catalase
superoxide dismutase

Keywords

  • Antioxidant enzymes
  • Human umbilical vein endothelial cells (HUVECs)
  • Malondialdehyde
  • Oxidative stress
  • Piper sarmentosum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • veterinary(all)

Cite this

Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide. / Hafizah, Abdul Hamid; Zakaria, Zaiton; Zulkhairi, Amom; Mohd Ilham, Adenan; Nor Anita, Megat Mohd Nordin; Abdullah Mahdy, Zaleha.

In: Journal of Zhejiang University: Science B, Vol. 11, No. 5, 05.2010, p. 357-365.

Research output: Contribution to journalArticle

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abstract = "Endothelial cell death due to increased reactive oxygen species (ROS) may contribute to the initial endothelial injury, which promotes atherosclerotic lesion formation. Piper sarmentosum (PS), a natural product, has been shown to have an antioxidant property, which is hypothesized to inhibit production of ROS and prevent cell injury. Thus, the present study was designed to determine the effects of PS on the hydrogen peroxide (H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells (HUVECs). In this experiment, HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation (LSGS). HUVECs were treated with various concentrations of H2O2 (01000 μmol/L) and it was observed that 180 μmol/L H2O2 reduced cell viability by 50{\%} as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Using the above concentration as the positive control, the H 2O2-induced HUVECs were concomitantly treated with various concentrations (100, 150, 250 and 300 μg/ml) of three different extracts (aqueous, methanol and hexane) of PS. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels showed a significant increase (P<0.05) in HUVECs compared to the negative control. However, PS extracts showed a protective effect on HUVECs from H 2O2-induced cell apoptosis with a significant reduction in MDA, SOD, CAT and GPX levels (P<0.05). Furthermore, PS had exhibited ferric reducing antioxidant power with its high phenolic content. Hence, it was concluded that PS plays a beneficial role in reducing oxidative stress in H 2O2-induced HUVECs.",
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AU - Mohd Ilham, Adenan

AU - Nor Anita, Megat Mohd Nordin

AU - Abdullah Mahdy, Zaleha

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AB - Endothelial cell death due to increased reactive oxygen species (ROS) may contribute to the initial endothelial injury, which promotes atherosclerotic lesion formation. Piper sarmentosum (PS), a natural product, has been shown to have an antioxidant property, which is hypothesized to inhibit production of ROS and prevent cell injury. Thus, the present study was designed to determine the effects of PS on the hydrogen peroxide (H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells (HUVECs). In this experiment, HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation (LSGS). HUVECs were treated with various concentrations of H2O2 (01000 μmol/L) and it was observed that 180 μmol/L H2O2 reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Using the above concentration as the positive control, the H 2O2-induced HUVECs were concomitantly treated with various concentrations (100, 150, 250 and 300 μg/ml) of three different extracts (aqueous, methanol and hexane) of PS. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels showed a significant increase (P<0.05) in HUVECs compared to the negative control. However, PS extracts showed a protective effect on HUVECs from H 2O2-induced cell apoptosis with a significant reduction in MDA, SOD, CAT and GPX levels (P<0.05). Furthermore, PS had exhibited ferric reducing antioxidant power with its high phenolic content. Hence, it was concluded that PS plays a beneficial role in reducing oxidative stress in H 2O2-induced HUVECs.

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