Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells

Pek Leng Ng, Nor Fadilah Rajab, Sue Mian Then, Yasmin Anum Mohd Yusof, Wan Zurinah Wan Ngah, Kar Yong Pin, Mee Lee Looi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: The combination effect of Piper betle (PB) and 5-fluorouracil (5-FU) in enhancing the cytotoxic potential of 5-FU in inhibiting the growth of colon cancer cells was investigated. Methods: HT29 and HCT116 cells were subjected to 5-FU or PB treatment. 5-FU and PB were then combined and their effects on both cell lines were observed after 24 h of treatment. PB-5-FU interaction was elucidated by isobologram analysis. Apoptosis features of the treated cells were revealed by annexin V/PI stain. High-performance liquid chromatography (HPLC) was performed to exclude any possible chemical interaction between the compounds. Results: In the presence of PB extract, the cytotoxicity of 5-FU was observed at a lower dose (IC50 12.5 μmol/L) and a shorter time (24 h) in both cell lines. Both cell lines treated with 5-FU or PB alone induced a greater apoptosis effect compared with the combination treatment. Isobologram analysis indicated that PB and 5-FU interacted synergistically and antagonistically in inhibiting the growth of HT29 and HCT116 cells, respectively. Conclusions: In the presence of PB, a lower dosage of 5-FU is required to achieve the maximum drug effect in inhibiting the growth of HT29 cells. However, PB did not significantly reduce 5-FU dosage in HCT116 cells. Our result showed that this interaction may not solely contribute to the apoptosis pathway.

Original languageEnglish
Pages (from-to)692-700
Number of pages9
JournalJournal of Zhejiang University: Science B
Volume15
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Piper betle
fluorouracil
Cytotoxicity
colorectal neoplasms
Fluorouracil
leaf extracts
Colonic Neoplasms
cytotoxicity
Cells
Growth
HCT116 Cells
HT29 Cells
apoptosis
cell lines
Apoptosis
Cell Line
dosage
neoplasm cells
cells
chemical interactions

Keywords

  • 5-Fluorouracil
  • Herb-drug interaction
  • Isobologram analysis
  • Piper betle L.
  • Piperaceae

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • veterinary(all)

Cite this

Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells. / Ng, Pek Leng; Rajab, Nor Fadilah; Then, Sue Mian; Mohd Yusof, Yasmin Anum; Wan Ngah, Wan Zurinah; Pin, Kar Yong; Looi, Mee Lee.

In: Journal of Zhejiang University: Science B, Vol. 15, No. 8, 2014, p. 692-700.

Research output: Contribution to journalArticle

Ng, Pek Leng ; Rajab, Nor Fadilah ; Then, Sue Mian ; Mohd Yusof, Yasmin Anum ; Wan Ngah, Wan Zurinah ; Pin, Kar Yong ; Looi, Mee Lee. / Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells. In: Journal of Zhejiang University: Science B. 2014 ; Vol. 15, No. 8. pp. 692-700.
@article{a324b5a3ccd24b3aad6a27859908065c,
title = "Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells",
abstract = "Objective: The combination effect of Piper betle (PB) and 5-fluorouracil (5-FU) in enhancing the cytotoxic potential of 5-FU in inhibiting the growth of colon cancer cells was investigated. Methods: HT29 and HCT116 cells were subjected to 5-FU or PB treatment. 5-FU and PB were then combined and their effects on both cell lines were observed after 24 h of treatment. PB-5-FU interaction was elucidated by isobologram analysis. Apoptosis features of the treated cells were revealed by annexin V/PI stain. High-performance liquid chromatography (HPLC) was performed to exclude any possible chemical interaction between the compounds. Results: In the presence of PB extract, the cytotoxicity of 5-FU was observed at a lower dose (IC50 12.5 μmol/L) and a shorter time (24 h) in both cell lines. Both cell lines treated with 5-FU or PB alone induced a greater apoptosis effect compared with the combination treatment. Isobologram analysis indicated that PB and 5-FU interacted synergistically and antagonistically in inhibiting the growth of HT29 and HCT116 cells, respectively. Conclusions: In the presence of PB, a lower dosage of 5-FU is required to achieve the maximum drug effect in inhibiting the growth of HT29 cells. However, PB did not significantly reduce 5-FU dosage in HCT116 cells. Our result showed that this interaction may not solely contribute to the apoptosis pathway.",
keywords = "5-Fluorouracil, Herb-drug interaction, Isobologram analysis, Piper betle L., Piperaceae",
author = "Ng, {Pek Leng} and Rajab, {Nor Fadilah} and Then, {Sue Mian} and {Mohd Yusof}, {Yasmin Anum} and {Wan Ngah}, {Wan Zurinah} and Pin, {Kar Yong} and Looi, {Mee Lee}",
year = "2014",
doi = "10.1631/jzus.B1300303",
language = "English",
volume = "15",
pages = "692--700",
journal = "Journal of Zhejiang University: Science B",
issn = "1673-1581",
publisher = "Zhejiang University Press",
number = "8",

}

TY - JOUR

T1 - Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells

AU - Ng, Pek Leng

AU - Rajab, Nor Fadilah

AU - Then, Sue Mian

AU - Mohd Yusof, Yasmin Anum

AU - Wan Ngah, Wan Zurinah

AU - Pin, Kar Yong

AU - Looi, Mee Lee

PY - 2014

Y1 - 2014

N2 - Objective: The combination effect of Piper betle (PB) and 5-fluorouracil (5-FU) in enhancing the cytotoxic potential of 5-FU in inhibiting the growth of colon cancer cells was investigated. Methods: HT29 and HCT116 cells were subjected to 5-FU or PB treatment. 5-FU and PB were then combined and their effects on both cell lines were observed after 24 h of treatment. PB-5-FU interaction was elucidated by isobologram analysis. Apoptosis features of the treated cells were revealed by annexin V/PI stain. High-performance liquid chromatography (HPLC) was performed to exclude any possible chemical interaction between the compounds. Results: In the presence of PB extract, the cytotoxicity of 5-FU was observed at a lower dose (IC50 12.5 μmol/L) and a shorter time (24 h) in both cell lines. Both cell lines treated with 5-FU or PB alone induced a greater apoptosis effect compared with the combination treatment. Isobologram analysis indicated that PB and 5-FU interacted synergistically and antagonistically in inhibiting the growth of HT29 and HCT116 cells, respectively. Conclusions: In the presence of PB, a lower dosage of 5-FU is required to achieve the maximum drug effect in inhibiting the growth of HT29 cells. However, PB did not significantly reduce 5-FU dosage in HCT116 cells. Our result showed that this interaction may not solely contribute to the apoptosis pathway.

AB - Objective: The combination effect of Piper betle (PB) and 5-fluorouracil (5-FU) in enhancing the cytotoxic potential of 5-FU in inhibiting the growth of colon cancer cells was investigated. Methods: HT29 and HCT116 cells were subjected to 5-FU or PB treatment. 5-FU and PB were then combined and their effects on both cell lines were observed after 24 h of treatment. PB-5-FU interaction was elucidated by isobologram analysis. Apoptosis features of the treated cells were revealed by annexin V/PI stain. High-performance liquid chromatography (HPLC) was performed to exclude any possible chemical interaction between the compounds. Results: In the presence of PB extract, the cytotoxicity of 5-FU was observed at a lower dose (IC50 12.5 μmol/L) and a shorter time (24 h) in both cell lines. Both cell lines treated with 5-FU or PB alone induced a greater apoptosis effect compared with the combination treatment. Isobologram analysis indicated that PB and 5-FU interacted synergistically and antagonistically in inhibiting the growth of HT29 and HCT116 cells, respectively. Conclusions: In the presence of PB, a lower dosage of 5-FU is required to achieve the maximum drug effect in inhibiting the growth of HT29 cells. However, PB did not significantly reduce 5-FU dosage in HCT116 cells. Our result showed that this interaction may not solely contribute to the apoptosis pathway.

KW - 5-Fluorouracil

KW - Herb-drug interaction

KW - Isobologram analysis

KW - Piper betle L.

KW - Piperaceae

UR - http://www.scopus.com/inward/record.url?scp=84903694918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903694918&partnerID=8YFLogxK

U2 - 10.1631/jzus.B1300303

DO - 10.1631/jzus.B1300303

M3 - Article

C2 - 25091987

AN - SCOPUS:84903694918

VL - 15

SP - 692

EP - 700

JO - Journal of Zhejiang University: Science B

JF - Journal of Zhejiang University: Science B

SN - 1673-1581

IS - 8

ER -