Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts

Lina Wati Durani, Shy Cian Khor, Jen Kit Tan, Kien Hui Chua, Yasmin Anum Mohd Yusof, Suzana Makpol

Research output: Research - peer-reviewArticle

Abstract

Piper betle (PB) is a traditional medicine that is widely used to treat different diseases around Asian region. The leaf extracts contain various bioactive compounds, which were reported to have antidiabetic, antibacterial, anti-inflammatory, antioxidant, and anticancer effects. In this study, the effect of PB aqueous extracts on replicative senescent human diploid fibroblasts (HDFs) was investigated by determining the expressions of senescence-associated genes using quantitative PCR. Our results showed that PB extracts at 0.4 mg/ml can improve cell proliferation of young (143%), presenescent (127.3%), and senescent (157.3%) HDFs. Increased expressions of PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were observed in senescent HDFs compared to young and/or presenescent HDFs. Treatment with PB extracts modulates the transcriptional profile changes in senescent HDFs. By contrast, expressions of SOD1 increased, whereas GPX1, PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were decreased in PB-treated senescent HDFs compared to untreated senescent HDFs. In conclusion, this study indicates the modulation of PB extracts on senescence-associated genes expression of replicative senescent HDFs. Further studies warrant determining the mechanism of PB in modulating replicative senescence of HDFs through these signaling pathways.

LanguageEnglish
Article number6894026
JournalBioMed Research International
Volume2017
DOIs
StatePublished - 2017

Fingerprint

Piper betle
Diploidy
Fibroblasts
Gene Expression
Gene expression
Mitogen-Activated Protein Kinase 14
Cell Aging
Traditional Medicine
Hypoglycemic Agents
Anti-Inflammatory Agents
Antioxidants
Cell Proliferation
Polymerase Chain Reaction
Genes
Therapeutics
Cell proliferation
Medicine
Modulation

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts. / Durani, Lina Wati; Khor, Shy Cian; Tan, Jen Kit; Chua, Kien Hui; Mohd Yusof, Yasmin Anum; Makpol, Suzana.

In: BioMed Research International, Vol. 2017, 6894026, 2017.

Research output: Research - peer-reviewArticle

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AU - Chua,Kien Hui

AU - Mohd Yusof,Yasmin Anum

AU - Makpol,Suzana

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