Piper betle Induced Cytoprotective Genes and Proteins via the Nrf2/ARE Pathway in Aging Mice

Nor Syahida Aliahmat, Nur Fathiah Abdul Sani, Wan Nuraini Wan Hasan, Suzana Makpol, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Aims: The objective of this study was to elucidate the underlying antioxidant mechanism of aqueous extract of Piper betle (PB) in aging rats. The nuclear factor erythroid 2-related factor 2 (Nrf2)/ARE pathway involving phase II detoxifying and antioxidant enzymes plays an important role in the antioxidant system by reducing electrophiles and reactive oxygen species through induction of phase II enzymes and proteins. Methods: Genes and proteins of phase II detoxifying antioxidant enzymes were analyzed by QuantiGenePlex 2.0 Assay and Western blot analysis. Results: PB significantly induced genes and proteins of phase II and antioxidant enzymes, NAD(P)H quinone oxidoreductase 1, and catalase in aging mice (p < 0.05). The expression of these enzymes were stimulated via translocation of Nrf2 into the nucleus, indicating the involvement of ARE, a cis-acting motif located in the promoter region of nearly all phase II genes. Conclusions: PB was testified for the first time to induce cytoprotective genes through the Nrf2/ARE signaling pathway, thus unraveling the antioxidant mechanism of PB during the aging process.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalJournal of Nutrigenetics and Nutrigenomics
DOIs
Publication statusAccepted/In press - 22 Dec 2016

Fingerprint

Piper betle
Antioxidants
antioxidants
mice
Enzymes
enzymes
Proteins
genes
proteins
NAD(P)H dehydrogenase (quinone)
Genetic Promoter Regions
NAD
Catalase
Genes
reactive oxygen species
Reactive Oxygen Species
catalase
Oxidoreductases
Western blotting
Western Blotting

Keywords

  • <italic>Piper betle</italic>
  • Aging rats
  • Cytoprotective genes
  • NAD(P)H quinone oxidoreductase 1
  • Nrf2/antioxidant-response element

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Genetics

Cite this

Aliahmat, N. S., Abdul Sani, N. F., Wan Hasan, W. N., Makpol, S., Wan Ngah, W. Z., & Mohd Yusof, Y. A. (Accepted/In press). Piper betle Induced Cytoprotective Genes and Proteins via the Nrf2/ARE Pathway in Aging Mice. Journal of Nutrigenetics and Nutrigenomics, 243-253. https://doi.org/10.1159/000452407

Piper betle Induced Cytoprotective Genes and Proteins via the Nrf2/ARE Pathway in Aging Mice. / Aliahmat, Nor Syahida; Abdul Sani, Nur Fathiah; Wan Hasan, Wan Nuraini; Makpol, Suzana; Wan Ngah, Wan Zurinah; Mohd Yusof, Yasmin Anum.

In: Journal of Nutrigenetics and Nutrigenomics, 22.12.2016, p. 243-253.

Research output: Contribution to journalArticle

Aliahmat, Nor Syahida ; Abdul Sani, Nur Fathiah ; Wan Hasan, Wan Nuraini ; Makpol, Suzana ; Wan Ngah, Wan Zurinah ; Mohd Yusof, Yasmin Anum. / Piper betle Induced Cytoprotective Genes and Proteins via the Nrf2/ARE Pathway in Aging Mice. In: Journal of Nutrigenetics and Nutrigenomics. 2016 ; pp. 243-253.
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