Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines

A. M. Alhassan, Q. U. Ahmed, Jalifah Latip, S. A.A. Shah, A'a Y.F. Khan, M. N. Sarian, R. A. Wahab, M. Taher, M. I. Abdullahi, A. Khatib

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Vernonia glaberrima is a medicinal plant that is used in African traditional medicine for the treatment of skin cancer. The aim of this study was to investigate the anticancer activity of V. glaberrima leaves and isolate its bioactive constituents. Crude methanolic leaves extract of V. glaberrima showing significant cytotoxic activity against cancer cell lines was subjected to chromatographic separation, purification and hydrolysis to yield four compounds namely, nonacosanoic acid, lupeol, 5-methylcoumarin-4-β-glucoside and 4-hydroxy-5-methylcoumarin. Three of the isolated compounds showed significant cytotoxic activity against human malignant melanoma (A375) cell line (IC50: 59.18 ± 2.70 to 139.53 ± 10.79 μg/mL) and human caucasian colon adenocarcinoma (HT-29) cell line (IC50: 4.22 ± 0.13 to 20.0 ± 1.91 μg/mL) while only lupeol displayed significant activity against human breast adenocarcinoma (MCF7) (IC50: 34.15 ± 2.32 μg/mL) cell line. CDK2 receptor and CAIX were identified through molecular docking as potential targets for the bioactive compounds. The findings of this study have revealed the therapeutic potential of V. glaberrima against breast cancer, skin cancer and colorectal carcinoma, respectively and further support its traditional uses in the treatment of skin cancer.

Original languageEnglish
Pages (from-to)16-24
Number of pages9
JournalSouth African Journal of Botany
Volume116
DOIs
Publication statusPublished - 1 May 2018

Fingerprint

chemical constituents of plants
Vernonia
cell lines
inhibitory concentration 50
lupeol
adenocarcinoma
leaves
melanoma
traditional medicine
colorectal neoplasms
leaf extracts
breast neoplasms
breasts
colon
glucosides
medicinal plants
hydrolysis
therapeutics
receptors
neoplasm cells

Keywords

  • 5-Methylcoumarin-4-β-glucoside
  • Anticancer activity
  • Carbonic anhydrase IX
  • Molecular docking
  • Vernonia glaberrima

ASJC Scopus subject areas

  • Plant Science

Cite this

Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines. / Alhassan, A. M.; Ahmed, Q. U.; Latip, Jalifah; Shah, S. A.A.; Khan, A'a Y.F.; Sarian, M. N.; Wahab, R. A.; Taher, M.; Abdullahi, M. I.; Khatib, A.

In: South African Journal of Botany, Vol. 116, 01.05.2018, p. 16-24.

Research output: Contribution to journalArticle

Alhassan, A. M. ; Ahmed, Q. U. ; Latip, Jalifah ; Shah, S. A.A. ; Khan, A'a Y.F. ; Sarian, M. N. ; Wahab, R. A. ; Taher, M. ; Abdullahi, M. I. ; Khatib, A. / Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines. In: South African Journal of Botany. 2018 ; Vol. 116. pp. 16-24.
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AU - Sarian, M. N.

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