Phytochemistry, antibacterial and antiviral effects of the fractions of Asplenium nidus leaves aqueous extract

Mariya Mohd Tahir, Nurain Shahera Hassan, Herryawan Ryadi Eziwar Dyari, Wan Yaacob Wan Ahmad, Nazlina Ibrahim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study the phytochemical content, antibacterial and antiviral potentials of Asplenium nidus leaves aqueous extract fractions was described. Leaves aqueous extract was fractionated using chloroform, hexane and ethyl acetate. Phytochemical screening revealed the presence of alkaloid, flavonoids and terpenoids in all fractions with anthraquinones available only in the ethyl acetate fraction. Safety of the fractions on Vero cells was determined from CC50 value i.e. the concentration that reduces 50% of cell viability. The fractions are not cytotoxic with CC50 value ranged from 0.78 to 32 mg/mL. The antibacterial activities of the fractions were evaluated against fifteen pathogenic bacteria by determining the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). The MIC and MBC values for the ethyl acetate fraction showed highest bactericidal activity against fourteen bacteria. The antibacterial selectivity indices (SI = CC50/MIC) for the fractions ranged between none to 40.94. The fractions have antiviral potential against Herpes Simplex Virus Type I (HSV-1) with effective concentration that reduces 50% of plaque formation (EC50) were between 0.056 to 0.54 mg/mL and selective index (SI = CC50/EC50) of the fractions ranged between 14 to 59. As a conclusion, fractions from the aqueous extract of A. nidus have potential as antibacterial and antiviral agents that may be attributed by the anthraquinones content.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalMalaysian Applied Biology
Volume46
Issue number1
Publication statusPublished - 1 Mar 2017

Fingerprint

Asplenium nidus
plant biochemistry
Antiviral Agents
Anthraquinones
anthraquinones
Phytochemicals
phytopharmaceuticals
extracts
antiviral agents
Bacteria
leaves
herpes simplex
Vero Cells
water
bacteria
Terpenes
Human Herpesvirus 1
Hexanes
Simplexvirus
Chloroform

Keywords

  • Anti HSV-1 activity
  • Asplenium nidus
  • Bactericidal activity
  • Cytotoxicity
  • Phytochemical content

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Phytochemistry, antibacterial and antiviral effects of the fractions of Asplenium nidus leaves aqueous extract. / Tahir, Mariya Mohd; Hassan, Nurain Shahera; Eziwar Dyari, Herryawan Ryadi; Wan Ahmad, Wan Yaacob; Ibrahim, Nazlina.

In: Malaysian Applied Biology, Vol. 46, No. 1, 01.03.2017, p. 207-212.

Research output: Contribution to journalArticle

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