Physicochemical properties and in vitro cytotoxicity studies of chitosan as a potential carrier for dicer-substrate siRNA

Maria Abdul Ghafoor Raja, Haliza Katas, Zariyantey Abd Hamid, Nur Atiqah Razali

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Recently, Dicer-substrate small interfering RNA (DsiRNA) has gained attention owing to its greater potency over small interfering RNA (siRNA). However, the use of DsiRNA is restricted by its rapid degradation in vitro. To address this issue, chitosan nanoparticulate deliver yplatform for the Dicer-substrate siRNA (DsiRNA) was developed and characterized. Nanoparticles were prepared by simple complexation and ionic gelation methods. The mean particle size of DsiRNA-adsorbed chitosan nanospheres (DsiRNA-CS NPs) prepared by the ionic gelation method ranged from 225 to 335 nm, while simple complexation yielded DsiRNA-chitosan complexes (DsiRNA-CS complexes) ranging from 270 to 730 nm. The zeta potential of both types of nanoparticles ranged from +40 to +65 mV. TEM and AFM micrographs revealed spherical and irregular morphology of DsiRNA-CS NPs and DsiRNA-CS complexes. ATR-FTIR spectroscopy confirmed the presence of DsiRNA in the CS NPs/complexes. Both types of nanoparticles exhibited sustained release and high binding and encapsulation (100%) efficiency of DsiRNA. DsiRNA-CS NPs/complexes showed low, concentration-dependent cytotoxicity in vitro. DsiRNA-CS NPs showed better stability than the complexes when stored at 4 and 25°C. Thus, it is anticipated that CS NPs are promising vectors for DsiRNA delivery due to their stability, safety, and cost-effectiveness.

Original languageEnglish
Article number653892
JournalJournal of Nanomaterials
Volume2013
DOIs
Publication statusPublished - 2013

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Chitosan
Cytotoxicity
RNA
Small Interfering RNA
Substrates
Gelation
Nanoparticles
Complexation
Nanospheres
Zeta potential
Cost effectiveness
Encapsulation
Particle size
Spectroscopy

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Physicochemical properties and in vitro cytotoxicity studies of chitosan as a potential carrier for dicer-substrate siRNA. / Abdul Ghafoor Raja, Maria; Katas, Haliza; Abd Hamid, Zariyantey; Razali, Nur Atiqah.

In: Journal of Nanomaterials, Vol. 2013, 653892, 2013.

Research output: Contribution to journalArticle

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