Physicochemical, cytotoxicity and genotoxicity assessment of palm oil mill effluent on V79 cells

N. Awang, S. N. Mustaffa, C. K. Meng

Research output: Contribution to journalArticle

Abstract

Palm oil mill effluent (POME) is the wastewater produced from palm oil refining activities like oil extraction, washing and cleaning processes in the mill that require an effective treatment before being discharged into waterways due to the effects it may have on the environment. The study was conducted in one of the mills that are located in Jerangau, Terengganu. This study was conducted to determine the physicochemical characteristics of POME on four different ponds which was mixing pond, facultative pond, algae pond and discharged pond. Discharged pond was the last pond for the POME treatment before it is discharged into the nearby river. The physicochemical parameters that have been determined to evaluate POME were pH, temperature, BOD, COD and TSS. The physicochemical characterization was carried out to determine the profile of POME at that time and the results showed that all parameters exceed the standards set by the Regulations of the Environmental Quality (Industrial Effluent) Regulations 2009 Standard B of the Environmental Quality Act 1974 except the temperature. In addition, this study also aimed to determine and compare the effects of cytotoxicity and genotoxicity of POME between the four ponds towards V79 cells. The cytotoxicity can be determined using MTT assay to evaluate the cytotoxic effect of POME on V79 cells. Meanwhile the genotoxicity was determined by using the alkaline comet assay to evaluate the genotoxic effect of POME on V79 cells. The study found that the POME in all ponds showed the cytotoxic effects on V79 cells and the POME in discharged pond showed the highest IC50 value compared to the other ponds which was 75% v/v. By using the IC25, the treatment towards V79 cells showed the value of the moments tail in the discharged pond was the lowest compared to the others which was 0.898 ± 0.561. The values of the tail moment are much lower when compared to the positive control which was 6.58 ± 1.383. Therefore, it can be concluded that the POME in a discharged pond had a lowest cytotoxic and genotoxic effects on V79 cells compared to other ponds.

Original languageEnglish
Pages (from-to)13523-13532
Number of pages10
JournalApplied Ecology and Environmental Research
Volume17
Issue number6
DOIs
Publication statusPublished - 1 Jan 2019

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oil mill effluents
genotoxicity
cytotoxicity
mill
pond
effluent
oil
cells
environmental quality
algae
tail
assay
oil refining
industrial effluents
waterways
biochemical oxygen demand
palm oils

Keywords

  • Genotoxicity
  • Palm oil mill effluent
  • Physicochemical characterizations
  • V79 cells

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science

Cite this

Physicochemical, cytotoxicity and genotoxicity assessment of palm oil mill effluent on V79 cells. / Awang, N.; Mustaffa, S. N.; Meng, C. K.

In: Applied Ecology and Environmental Research, Vol. 17, No. 6, 01.01.2019, p. 13523-13532.

Research output: Contribution to journalArticle

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