Physicochemical characterization and stability studies of duck Pasteurellosis nanovaccine using palm oil (MCT-LCT) lipid emulsion as adjuvant

Research output: Contribution to journalArticle

Abstract

Physicochemical stability is very important for vaccines manufacturing, distribution and administration, especially in tropical and developing countries where particularly adverse field conditions exist. Stability of a vaccine formulation is important when the adjuvant used is made up of different oil emulsions. Various oils (mineral and non-mineral) and materials used as adjuvants have been shown to produce adversity and toxic effects. An inactivated duck Pasteurellosis nanovaccine was prepared by using a combination of palm oil/coconut oil (50: 50) as a novel adjuvant. The vaccine was placed at different temperatures (4°C, 25°C and 40°C) and the physicochemical changes were measured for the stability of the vaccine. Six-month physicochemical stability study results show no significant change in the observed parameters of formulation. Globule size (252 nm Z-Average d. nm), zeta potential (-11.04 mV), pH (5.7), viscosity (52.0 cps) were measured and gas chromatographic analysis shows no significant change in the total fatty acid content of the vaccine. ATR-FTIR was performed and formulations were scanned from the range of 4000 to 500 cm-1. All peaks indicating presence of palm oil, coconut oil and Pasteurella multocida (antigen) were observed, indicating the stable state condition and there is no chemical change in the formulation which means the nano vaccine is physicochemically stable under these conditions.

Original languageEnglish
Pages (from-to)1167-1175
Number of pages9
JournalActa Poloniae Pharmaceutica - Drug Research
Volume74
Issue number4
Publication statusPublished - 2017

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Pasteurella Infections
Ducks
Emulsions
Vaccines
Vaccine Potency
Lipids
Pasteurella multocida
Mineral Oil
Poisons
Fourier Transform Infrared Spectroscopy
Viscosity
Gas Chromatography
Developing Countries
Oils
Fatty Acids
Antigens
Temperature
palm oil

Keywords

  • Coconut oil
  • Nanovaccine
  • Palm oil
  • Pasteurella multocida
  • Physico-chemical stability

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

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title = "Physicochemical characterization and stability studies of duck Pasteurellosis nanovaccine using palm oil (MCT-LCT) lipid emulsion as adjuvant",
abstract = "Physicochemical stability is very important for vaccines manufacturing, distribution and administration, especially in tropical and developing countries where particularly adverse field conditions exist. Stability of a vaccine formulation is important when the adjuvant used is made up of different oil emulsions. Various oils (mineral and non-mineral) and materials used as adjuvants have been shown to produce adversity and toxic effects. An inactivated duck Pasteurellosis nanovaccine was prepared by using a combination of palm oil/coconut oil (50: 50) as a novel adjuvant. The vaccine was placed at different temperatures (4°C, 25°C and 40°C) and the physicochemical changes were measured for the stability of the vaccine. Six-month physicochemical stability study results show no significant change in the observed parameters of formulation. Globule size (252 nm Z-Average d. nm), zeta potential (-11.04 mV), pH (5.7), viscosity (52.0 cps) were measured and gas chromatographic analysis shows no significant change in the total fatty acid content of the vaccine. ATR-FTIR was performed and formulations were scanned from the range of 4000 to 500 cm-1. All peaks indicating presence of palm oil, coconut oil and Pasteurella multocida (antigen) were observed, indicating the stable state condition and there is no chemical change in the formulation which means the nano vaccine is physicochemically stable under these conditions.",
keywords = "Coconut oil, Nanovaccine, Palm oil, Pasteurella multocida, Physico-chemical stability",
author = "Mufti, {Attique Ur Rahman} and Endang, {Kumolosasi Msi} and Shamsuddin, {Ahmad Fuad}",
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AU - Endang, Kumolosasi Msi

AU - Shamsuddin, Ahmad Fuad

PY - 2017

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