Physicochemical characteristics of attached biofilm on granular activated carbon for thermophilic biohydrogen production

Nabilah Aminah Lutpi, Jamaliah Md Jahim, Tabassum Mumtaz, Peer Mohamed Abdul, Mohd Tusirin Mohd Nor

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this study, thermophilic biohydrogen production by a mixed culture, obtained from a continuous acidogenic reactor treating palm oil mill effluent, was improved by using granular activated carbon (GAC) as the support material. Batch experiments were carried out at 60 °C by feeding the anaerobic sludge bacteria with a sucrose-containing synthetic medium at an initial pH of 5.5 under anoxic conditions. The physico-chemical characteristics of the attached biofilm were evaluated after extraction of the extracellular polymeric substances (EPSs) of the biofilm using the formaldehyde-NaOH method. The main component of the biofilm was protein (60%), while the carbohydrate content accounted for 40% of the EPS. Two major absorption bands at approximately 3400 cm-1 and 1650 cm-1, characteristics of the stretching vibrations of hydroxyl and amino groups, respectively, were identified in the FT-IR spectra, confirming the composition of the EPS. Observations using scanning electron microscopy (SEM) illustrated the attachment of rod-shaped bacterial cells on the GAC at 60 °C. A maximum hydrogen production rate of 4.3 mmol L-1 h-1 and a hydrogen yield of 5.6 mol H2 per mol sucrose were obtained from this attached biofilm system. The major soluble metabolites of fermentation were acetic acid and butyric acid. The results showed that the granular activated carbon enhanced the biohydrogen production by stabilizing the pH and microbial metabolites and therefore could be used as a support material for fermentative hydrogen production under thermophilic conditions on a large scale. This journal is

Original languageEnglish
Pages (from-to)19382-19392
Number of pages11
JournalRSC Advances
Volume5
Issue number25
DOIs
Publication statusPublished - 2015

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Biofilms
Activated carbon
Sugar (sucrose)
Hydrogen production
Metabolites
Sucrose
Palm oil
Butyric acid
Butyric Acid
Carbohydrates
Catalyst supports
Formaldehyde
Acetic acid
Acetic Acid
Hydroxyl Radical
Fermentation
Stretching
Absorption spectra
Effluents
Hydrogen

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Physicochemical characteristics of attached biofilm on granular activated carbon for thermophilic biohydrogen production. / Lutpi, Nabilah Aminah; Md Jahim, Jamaliah; Mumtaz, Tabassum; Abdul, Peer Mohamed; Mohd Nor, Mohd Tusirin.

In: RSC Advances, Vol. 5, No. 25, 2015, p. 19382-19392.

Research output: Contribution to journalArticle

Lutpi, Nabilah Aminah ; Md Jahim, Jamaliah ; Mumtaz, Tabassum ; Abdul, Peer Mohamed ; Mohd Nor, Mohd Tusirin. / Physicochemical characteristics of attached biofilm on granular activated carbon for thermophilic biohydrogen production. In: RSC Advances. 2015 ; Vol. 5, No. 25. pp. 19382-19392.
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