Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro

Nurulain ‘Atikah Kamalaldin, Mariatti Jaafar, Saiful Irwan Zubairi, Badrul Hisham Yahaya

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The use of bioceramics, especially the combination of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), as a three-dimensional scaffold in bone engineering is essential because together these elements constitute 60% of the bone content. Different ratios of HA and β-TCP were previously tested for their ability to produce suitable bioceramic scaffolds, which must be able to withstand high mechanical load. In this study, two ratios of HA/TCP (20:80 and 70:30) were used to create pellets, which then were evaluated in vitro to identify any adverse effects of using the material in bone grafting. Diametral tensile strength (DTS) and density testing was conducted to assess the mechanical strength and porosity of the pellets. The pellets then were tested for their toxicity to normal human fibroblast cells. In the toxicity assay, cells were incubated with the pellets for 3days. At the end of the experiment, cell morphological changes were assessed, and the absorbance was read using PrestoBlue Cell Viability Reagent™. An inversely proportional relationship between DTS and porosity percentage was detected. Fibroblasts showed normal cell morphology in both treatments, which suggests that the HA/TCP pellets were not toxic. In the osteoblast cell attachment assay, cells were able to attach to the surface of both ratios, but cells were also able to penetrate inside the scaffold of the 70:30 pellets. This finding suggests that the 70:30 ratio had better osteoconduction properties than the 20:80 ratio.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages1-15
Number of pages15
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1084
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Fingerprint

Durapatite
Scaffolds
Bioceramics
Mechanical properties
Bone
Fibroblasts
Toxicity
Assays
Tensile strength
Porosity
Cells
Tensile Strength
Poisons
Osteoblasts
Strength of materials
Bone and Bones
Bone Regeneration
Bone Transplantation
In Vitro Techniques
Testing

Keywords

  • 3D scaffold
  • Bioceramics
  • Bone substitution
  • Cell evaluation
  • Cell toxicity
  • HA/TCP

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kamalaldin, N. A., Jaafar, M., Zubairi, S. I., & Yahaya, B. H. (2019). Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro. In Advances in Experimental Medicine and Biology (pp. 1-15). (Advances in Experimental Medicine and Biology; Vol. 1084). Springer New York LLC. https://doi.org/10.1007/5584_2017_130

Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro. / Kamalaldin, Nurulain ‘Atikah; Jaafar, Mariatti; Zubairi, Saiful Irwan; Yahaya, Badrul Hisham.

Advances in Experimental Medicine and Biology. Springer New York LLC, 2019. p. 1-15 (Advances in Experimental Medicine and Biology; Vol. 1084).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kamalaldin, NA, Jaafar, M, Zubairi, SI & Yahaya, BH 2019, Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro. in Advances in Experimental Medicine and Biology. Advances in Experimental Medicine and Biology, vol. 1084, Springer New York LLC, pp. 1-15. https://doi.org/10.1007/5584_2017_130
Kamalaldin NA, Jaafar M, Zubairi SI, Yahaya BH. Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro. In Advances in Experimental Medicine and Biology. Springer New York LLC. 2019. p. 1-15. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/5584_2017_130
Kamalaldin, Nurulain ‘Atikah ; Jaafar, Mariatti ; Zubairi, Saiful Irwan ; Yahaya, Badrul Hisham. / Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro. Advances in Experimental Medicine and Biology. Springer New York LLC, 2019. pp. 1-15 (Advances in Experimental Medicine and Biology).
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