Phyltetralin, 1,7,8-trihydroxy 2-naphtaldehyde, ethyl 8-hydroxy-8-methyl-tridecanoate and 1-triacontanol from Phyllanthus amarus Schumach. & Thonn. inhibit phagocytic activity of human leucocytes

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the immunosuppressive effects of ethyl 8-hydroxy-8-methyl-tridecanoate, 1-triacontanol, phyltetralin and 1,7,8-trihydroxy 2-naphtaldehyde isolated from Phyllanthus amarus Schumach. & Thonn. on chemotaxis, oxidative burst, CD18 complex expression and phagocytosis ability of human leucocytes. Methods: Polymorphonuclear neutrophil (PMN) migration activity was measured using a modified Boyden chamber, and inhibition on oxidative burst was determined by chemiluminescence assay. The effects on CD18 complex expression and phagocytosis ability were evaluated by flow cytometry. Key findings: All compounds tested exhibited significant inhibitory effects on chemotaxis and respiratory burst. Among the compounds tested, phyltetralin demonstrated the highest inhibition on PMN chemotaxis with an IC50 value of 4.01 µm. Phyltetralin and 1,7,8-trihydroxy 2-naphtaldehyde showed strong inhibition against reactive oxygen species (ROS) production from PMA-stimulated neutrophil with IC50 values of 0.73 and 0.79 µm, respectively, which were comparable to that of aspirin (0.77 µm). All compounds showed weak inhibition on CD18 expression and moderate inhibition on engulfment activity. Conclusion: Compounds isolated from P. amarus possessed immunosuppressive effects on different lineages of innate immune system.

Original languageEnglish
JournalJournal of Pharmacy and Pharmacology
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Phyllanthus
Human Activities
Leukocytes
Respiratory Burst
Chemotaxis
Neutrophils
Aptitude
Immunosuppressive Agents
Phagocytosis
Inhibitory Concentration 50
Luminescence
Aspirin
Immune System
Reactive Oxygen Species
Flow Cytometry
Inhibition (Psychology)
1-triacontanol

Keywords

  • 1,7,8-trihydroxy 2-naphtaldehyde
  • 1-triacontanol
  • ethyl 8-hydroxy-8-methyl-tridecanoate
  • immunosuppressive effects
  • Phyllanthus amarus Schumach. & Thonn.
  • phyltetralin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

@article{c49c8c0c9b8d4bac8f8e51923414fcc4,
title = "Phyltetralin, 1,7,8-trihydroxy 2-naphtaldehyde, ethyl 8-hydroxy-8-methyl-tridecanoate and 1-triacontanol from Phyllanthus amarus Schumach. & Thonn. inhibit phagocytic activity of human leucocytes",
abstract = "Objective: To investigate the immunosuppressive effects of ethyl 8-hydroxy-8-methyl-tridecanoate, 1-triacontanol, phyltetralin and 1,7,8-trihydroxy 2-naphtaldehyde isolated from Phyllanthus amarus Schumach. & Thonn. on chemotaxis, oxidative burst, CD18 complex expression and phagocytosis ability of human leucocytes. Methods: Polymorphonuclear neutrophil (PMN) migration activity was measured using a modified Boyden chamber, and inhibition on oxidative burst was determined by chemiluminescence assay. The effects on CD18 complex expression and phagocytosis ability were evaluated by flow cytometry. Key findings: All compounds tested exhibited significant inhibitory effects on chemotaxis and respiratory burst. Among the compounds tested, phyltetralin demonstrated the highest inhibition on PMN chemotaxis with an IC50 value of 4.01 µm. Phyltetralin and 1,7,8-trihydroxy 2-naphtaldehyde showed strong inhibition against reactive oxygen species (ROS) production from PMA-stimulated neutrophil with IC50 values of 0.73 and 0.79 µm, respectively, which were comparable to that of aspirin (0.77 µm). All compounds showed weak inhibition on CD18 expression and moderate inhibition on engulfment activity. Conclusion: Compounds isolated from P. amarus possessed immunosuppressive effects on different lineages of innate immune system.",
keywords = "1,7,8-trihydroxy 2-naphtaldehyde, 1-triacontanol, ethyl 8-hydroxy-8-methyl-tridecanoate, immunosuppressive effects, Phyllanthus amarus Schumach. & Thonn., phyltetralin",
author = "Yuandani and Ibrahim Jantan and Khairana Husain",
year = "2019",
month = "1",
day = "1",
doi = "10.1111/jphp.13139",
language = "English",
journal = "Journal of Pharmacy and Pharmacology",
issn = "0022-3573",
publisher = "Pharmaceutical Press",

}

TY - JOUR

T1 - Phyltetralin, 1,7,8-trihydroxy 2-naphtaldehyde, ethyl 8-hydroxy-8-methyl-tridecanoate and 1-triacontanol from Phyllanthus amarus Schumach. & Thonn. inhibit phagocytic activity of human leucocytes

AU - Yuandani,

AU - Jantan, Ibrahim

AU - Husain, Khairana

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objective: To investigate the immunosuppressive effects of ethyl 8-hydroxy-8-methyl-tridecanoate, 1-triacontanol, phyltetralin and 1,7,8-trihydroxy 2-naphtaldehyde isolated from Phyllanthus amarus Schumach. & Thonn. on chemotaxis, oxidative burst, CD18 complex expression and phagocytosis ability of human leucocytes. Methods: Polymorphonuclear neutrophil (PMN) migration activity was measured using a modified Boyden chamber, and inhibition on oxidative burst was determined by chemiluminescence assay. The effects on CD18 complex expression and phagocytosis ability were evaluated by flow cytometry. Key findings: All compounds tested exhibited significant inhibitory effects on chemotaxis and respiratory burst. Among the compounds tested, phyltetralin demonstrated the highest inhibition on PMN chemotaxis with an IC50 value of 4.01 µm. Phyltetralin and 1,7,8-trihydroxy 2-naphtaldehyde showed strong inhibition against reactive oxygen species (ROS) production from PMA-stimulated neutrophil with IC50 values of 0.73 and 0.79 µm, respectively, which were comparable to that of aspirin (0.77 µm). All compounds showed weak inhibition on CD18 expression and moderate inhibition on engulfment activity. Conclusion: Compounds isolated from P. amarus possessed immunosuppressive effects on different lineages of innate immune system.

AB - Objective: To investigate the immunosuppressive effects of ethyl 8-hydroxy-8-methyl-tridecanoate, 1-triacontanol, phyltetralin and 1,7,8-trihydroxy 2-naphtaldehyde isolated from Phyllanthus amarus Schumach. & Thonn. on chemotaxis, oxidative burst, CD18 complex expression and phagocytosis ability of human leucocytes. Methods: Polymorphonuclear neutrophil (PMN) migration activity was measured using a modified Boyden chamber, and inhibition on oxidative burst was determined by chemiluminescence assay. The effects on CD18 complex expression and phagocytosis ability were evaluated by flow cytometry. Key findings: All compounds tested exhibited significant inhibitory effects on chemotaxis and respiratory burst. Among the compounds tested, phyltetralin demonstrated the highest inhibition on PMN chemotaxis with an IC50 value of 4.01 µm. Phyltetralin and 1,7,8-trihydroxy 2-naphtaldehyde showed strong inhibition against reactive oxygen species (ROS) production from PMA-stimulated neutrophil with IC50 values of 0.73 and 0.79 µm, respectively, which were comparable to that of aspirin (0.77 µm). All compounds showed weak inhibition on CD18 expression and moderate inhibition on engulfment activity. Conclusion: Compounds isolated from P. amarus possessed immunosuppressive effects on different lineages of innate immune system.

KW - 1,7,8-trihydroxy 2-naphtaldehyde

KW - 1-triacontanol

KW - ethyl 8-hydroxy-8-methyl-tridecanoate

KW - immunosuppressive effects

KW - Phyllanthus amarus Schumach. & Thonn.

KW - phyltetralin

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U2 - 10.1111/jphp.13139

DO - 10.1111/jphp.13139

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JO - Journal of Pharmacy and Pharmacology

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SN - 0022-3573

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