Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences

N. J. Ibrahim, S. Shariff, Idris Abd. Ghani, Badrul Munir Md. Zain, Y. Suhana, M. N. Roff, Salmah Yaakop

Research output: Contribution to journalArticle

Abstract

Opiinae (Hymenoptera: Braconidae) is an important parasitoid of fruit flies (Diptera: Tephritidae). Accurate identification is needed for them to be effectively used as biological control agents. This study demonstrates the use of molecular phylogenetic analyses to reconfirm the species of Opiinae reared from tephritids infesting star fruit species. A total of 14 specimens were isolated and a 401 bp of mitochondrial 16S rRNA gene was amplified and sequenced from each of them. Three individual tephritids hosts (Bactrocera papayae) were also identified based on the mitochondrial ND1 gene. Maximum Parsimony (MP) tree was constructed using PAUP 4.0b10. Three species of Opiinae were successfully identified based on the mitochondrial 16S rRNA gene, namely Diachasmimorpha longicaudata (Ashmead), Fopius arisanus (Sonan), and Psyttalia incisi (Silvestri). These molecular-based findings reconfirmed the parasitoid species of B. papayae as recorded by previous studies based on morphology. In fact, the identification of Opiinae individuals have been reconfirmed based on topology and branching pattern of the phylogenetic tree as well as based on genetic distance analyses, which matched morphological-based identification.

Original languageEnglish
Pages (from-to)345-358
Number of pages14
JournalPertanika Journal of Tropical Agricultural Science
Volume36
Issue number4
Publication statusPublished - Nov 2013

Fingerprint

Bactrocera papayae
Tephritidae
Averrhoa carambola
fruit flies
Fruits
Braconidae
parasitoid
rRNA Genes
Diptera
Stars
Fruit
fruit
Genes
ribosomal RNA
Biological Control Agents
phylogenetics
Hymenoptera
Mitochondrial Genes
phylogeny
Psyttalia

Keywords

  • 16S
  • Bactrocera papayae
  • Opiinae
  • Parasitoid
  • Rearing
  • Star fruit

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Agricultural and Biological Sciences(all)
  • Chemical Engineering(all)
  • Computer Science(all)
  • Environmental Science(all)

Cite this

@article{7e483cf158e6415a8e1675aebbce4ee4,
title = "Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences",
abstract = "Opiinae (Hymenoptera: Braconidae) is an important parasitoid of fruit flies (Diptera: Tephritidae). Accurate identification is needed for them to be effectively used as biological control agents. This study demonstrates the use of molecular phylogenetic analyses to reconfirm the species of Opiinae reared from tephritids infesting star fruit species. A total of 14 specimens were isolated and a 401 bp of mitochondrial 16S rRNA gene was amplified and sequenced from each of them. Three individual tephritids hosts (Bactrocera papayae) were also identified based on the mitochondrial ND1 gene. Maximum Parsimony (MP) tree was constructed using PAUP 4.0b10. Three species of Opiinae were successfully identified based on the mitochondrial 16S rRNA gene, namely Diachasmimorpha longicaudata (Ashmead), Fopius arisanus (Sonan), and Psyttalia incisi (Silvestri). These molecular-based findings reconfirmed the parasitoid species of B. papayae as recorded by previous studies based on morphology. In fact, the identification of Opiinae individuals have been reconfirmed based on topology and branching pattern of the phylogenetic tree as well as based on genetic distance analyses, which matched morphological-based identification.",
keywords = "16S, Bactrocera papayae, Opiinae, Parasitoid, Rearing, Star fruit",
author = "Ibrahim, {N. J.} and S. Shariff and {Abd. Ghani}, Idris and {Md. Zain}, {Badrul Munir} and Y. Suhana and Roff, {M. N.} and Salmah Yaakop",
year = "2013",
month = "11",
language = "English",
volume = "36",
pages = "345--358",
journal = "Pertanika Journal of Tropical Agricultural Science",
issn = "1511-3701",
publisher = "Universiti Putra Malaysia",
number = "4",

}

TY - JOUR

T1 - Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae

T2 - Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences

AU - Ibrahim, N. J.

AU - Shariff, S.

AU - Abd. Ghani, Idris

AU - Md. Zain, Badrul Munir

AU - Suhana, Y.

AU - Roff, M. N.

AU - Yaakop, Salmah

PY - 2013/11

Y1 - 2013/11

N2 - Opiinae (Hymenoptera: Braconidae) is an important parasitoid of fruit flies (Diptera: Tephritidae). Accurate identification is needed for them to be effectively used as biological control agents. This study demonstrates the use of molecular phylogenetic analyses to reconfirm the species of Opiinae reared from tephritids infesting star fruit species. A total of 14 specimens were isolated and a 401 bp of mitochondrial 16S rRNA gene was amplified and sequenced from each of them. Three individual tephritids hosts (Bactrocera papayae) were also identified based on the mitochondrial ND1 gene. Maximum Parsimony (MP) tree was constructed using PAUP 4.0b10. Three species of Opiinae were successfully identified based on the mitochondrial 16S rRNA gene, namely Diachasmimorpha longicaudata (Ashmead), Fopius arisanus (Sonan), and Psyttalia incisi (Silvestri). These molecular-based findings reconfirmed the parasitoid species of B. papayae as recorded by previous studies based on morphology. In fact, the identification of Opiinae individuals have been reconfirmed based on topology and branching pattern of the phylogenetic tree as well as based on genetic distance analyses, which matched morphological-based identification.

AB - Opiinae (Hymenoptera: Braconidae) is an important parasitoid of fruit flies (Diptera: Tephritidae). Accurate identification is needed for them to be effectively used as biological control agents. This study demonstrates the use of molecular phylogenetic analyses to reconfirm the species of Opiinae reared from tephritids infesting star fruit species. A total of 14 specimens were isolated and a 401 bp of mitochondrial 16S rRNA gene was amplified and sequenced from each of them. Three individual tephritids hosts (Bactrocera papayae) were also identified based on the mitochondrial ND1 gene. Maximum Parsimony (MP) tree was constructed using PAUP 4.0b10. Three species of Opiinae were successfully identified based on the mitochondrial 16S rRNA gene, namely Diachasmimorpha longicaudata (Ashmead), Fopius arisanus (Sonan), and Psyttalia incisi (Silvestri). These molecular-based findings reconfirmed the parasitoid species of B. papayae as recorded by previous studies based on morphology. In fact, the identification of Opiinae individuals have been reconfirmed based on topology and branching pattern of the phylogenetic tree as well as based on genetic distance analyses, which matched morphological-based identification.

KW - 16S

KW - Bactrocera papayae

KW - Opiinae

KW - Parasitoid

KW - Rearing

KW - Star fruit

UR - http://www.scopus.com/inward/record.url?scp=84893367133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893367133&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84893367133

VL - 36

SP - 345

EP - 358

JO - Pertanika Journal of Tropical Agricultural Science

JF - Pertanika Journal of Tropical Agricultural Science

SN - 1511-3701

IS - 4

ER -