Phylogenetic relationships of Malaysia's long-tailed macaques, Macaca fascicularis, based on cytochrome b sequences

Muhammad Abu Bakar Abdul-Latiff, Farhani Ruslin, Vun Vui Fui, Mohd Hashim Abu, Jeffrine Japning Rovie-Ryan, Pazil Abdul-Patah, Maklarin Lakim, Christian Roos, Salmah Yaakop, Badrul Munir Md. Zain

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Phylogenetic relationships among Malaysia's long-tailed macaques have yet to be established, despite abundant genetic studies of the species worldwide. The aims of this study are to examine the phylogenetic relationships of Macaca fascicularis in Malaysia and to test its classification as a morphological subspecies. A total of 25 genetic samples of M. fascicularis yielding 383 bp of Cytochrome b (Cyt b) sequences were used in phylogenetic analysis along with one sample each of M. nemestrina and M. arctoides used as outgroups. Sequence character analysis reveals that Cyt b locus is a highly conserved region with only 23% parsimony informative character detected among ingroups. Further analysis indicates a clear separation between populations originating from different regions; the Malay Peninsula versus Borneo Insular, the East Coast versus West Coast of the Malay Peninsula, and the island versus mainland Malay Peninsula populations. Phylogenetic trees (NJ, MP and Bayesian) portray a consistent clustering paradigm as Borneo's population was distinguished from Peninsula's population (99% and 100% bootstrap value in NJ and MP respectively and 1.00 posterior probability in Bayesian trees). The East coast population was separated from other Peninsula populations (64% in NJ, 66% in MP and 0.53 posterior probability in Bayesian). West coast populations were divided into 2 clades: the North-South (47%/54% in NJ, 26/26% in MP and 1.00/0.80 posterior probability in Bayesian) and Island-Mainland (93% in NJ, 90% in MP and 1.00 posterior probability in Bayesian). The results confirm the previous morphological assignment of 2 subspecies, M. f. fascicularis and M. f. argentimembris, in the Malay Peninsula. These populations should be treated as separate genetic entities in order to conserve the genetic diversity of Malaysia's M. fascicularis. These findings are crucial in aiding the conservation management and translocation process of M. fascicularis populations in Malaysia.

Original languageEnglish
Pages (from-to)121-139
Number of pages19
JournalZooKeys
Volume407
DOIs
Publication statusPublished - 2014

Fingerprint

Macaca fascicularis
Macaca
cytochrome b
Malaysia
cytochrome
phylogenetics
coast
phylogeny
subspecies
coasts
conservation management
translocation
Borneo
analysis
taxonomy
sampling
genetic variation
loci

Keywords

  • Cytochrome b
  • Long-tailed macaque
  • Macaca fascicularis
  • Phylogenetic relationships

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Phylogenetic relationships of Malaysia's long-tailed macaques, Macaca fascicularis, based on cytochrome b sequences. / Abdul-Latiff, Muhammad Abu Bakar; Ruslin, Farhani; Fui, Vun Vui; Abu, Mohd Hashim; Rovie-Ryan, Jeffrine Japning; Abdul-Patah, Pazil; Lakim, Maklarin; Roos, Christian; Yaakop, Salmah; Md. Zain, Badrul Munir.

In: ZooKeys, Vol. 407, 2014, p. 121-139.

Research output: Contribution to journalArticle

Abdul-Latiff, MAB, Ruslin, F, Fui, VV, Abu, MH, Rovie-Ryan, JJ, Abdul-Patah, P, Lakim, M, Roos, C, Yaakop, S & Md. Zain, BM 2014, 'Phylogenetic relationships of Malaysia's long-tailed macaques, Macaca fascicularis, based on cytochrome b sequences', ZooKeys, vol. 407, pp. 121-139. https://doi.org/10.3897/zookeys.407.6982
Abdul-Latiff, Muhammad Abu Bakar ; Ruslin, Farhani ; Fui, Vun Vui ; Abu, Mohd Hashim ; Rovie-Ryan, Jeffrine Japning ; Abdul-Patah, Pazil ; Lakim, Maklarin ; Roos, Christian ; Yaakop, Salmah ; Md. Zain, Badrul Munir. / Phylogenetic relationships of Malaysia's long-tailed macaques, Macaca fascicularis, based on cytochrome b sequences. In: ZooKeys. 2014 ; Vol. 407. pp. 121-139.
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