Perbandingan filogenetik protein antigen-I yang berpotensi sebagai calon diagnostik dan vaksin terhadap parasit Cryptocaryon irritans

Translated title of the contribution: Phylogenetic comparison of I-antigens protein as potential candidate for diagnostic and vaccine for Cryptocaryon irritans parasite

Research output: Contribution to journalArticle

Abstract

The fish parasite C. irritans i-antigen protein has high potential to be developed as a commercial vaccine against C. irritans. However, variation within the i-antigens of different C. irritans serotypes affected the cross-protection provided towards different isolates of C irritans when the i-antigen was evaluated as a candidate vaccine. This study was conducted to compare the sequences of various C. irritans i-antigens isolated in Malaysia with i-antigen sequences of previously reported C. irritans isolates. Phylogenetic comparison was performed to predict the protein's potential to be developed as a screening marker and vaccine candidate against various C. irritans isolates. Multiple sequence alignment of i-antigen amino acid sequences was undertaken using ClustalX and phylogenetic analysis of i-antigen was performed using the maximum parsimony (MP) and Bayesian methods. Nine C. irritans unique transcripts (UTs) had significant matches with i-antigen sequences in the NCBI protein database, with similarities ranging between 41 and 71%. Both MP and Bayesian trees generated showed that i-antigens cn56 and cn57 variants clustered together in one group while other i-antigen variants were divided over two different groups and the grouping was supported by the presence of conserved amino acids in their respective groups. Further research should identify variants of the i-antigen suitable for serodiagnostic candidates and provision of cross-protection against multiple strains of C irritans globally.

Original languageIndonesian
Pages (from-to)1969-1979
Number of pages11
JournalSains Malaysiana
Volume45
Issue number12
Publication statusPublished - 1 Dec 2016

Fingerprint

Parasites
Vaccines
Antigens
Proteins
Cross Protection
Marker Vaccines
I-antigen
Protein Databases
Bayes Theorem
Sequence Alignment
Malaysia
Amino Acid Sequence
Fishes
Amino Acids
Research

Keywords

  • Conformation
  • Cross protection
  • Cryptocaryon irritans
  • I-antigen
  • Serotype

ASJC Scopus subject areas

  • General

Cite this

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title = "Perbandingan filogenetik protein antigen-I yang berpotensi sebagai calon diagnostik dan vaksin terhadap parasit Cryptocaryon irritans",
abstract = "The fish parasite C. irritans i-antigen protein has high potential to be developed as a commercial vaccine against C. irritans. However, variation within the i-antigens of different C. irritans serotypes affected the cross-protection provided towards different isolates of C irritans when the i-antigen was evaluated as a candidate vaccine. This study was conducted to compare the sequences of various C. irritans i-antigens isolated in Malaysia with i-antigen sequences of previously reported C. irritans isolates. Phylogenetic comparison was performed to predict the protein's potential to be developed as a screening marker and vaccine candidate against various C. irritans isolates. Multiple sequence alignment of i-antigen amino acid sequences was undertaken using ClustalX and phylogenetic analysis of i-antigen was performed using the maximum parsimony (MP) and Bayesian methods. Nine C. irritans unique transcripts (UTs) had significant matches with i-antigen sequences in the NCBI protein database, with similarities ranging between 41 and 71{\%}. Both MP and Bayesian trees generated showed that i-antigens cn56 and cn57 variants clustered together in one group while other i-antigen variants were divided over two different groups and the grouping was supported by the presence of conserved amino acids in their respective groups. Further research should identify variants of the i-antigen suitable for serodiagnostic candidates and provision of cross-protection against multiple strains of C irritans globally.",
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AB - The fish parasite C. irritans i-antigen protein has high potential to be developed as a commercial vaccine against C. irritans. However, variation within the i-antigens of different C. irritans serotypes affected the cross-protection provided towards different isolates of C irritans when the i-antigen was evaluated as a candidate vaccine. This study was conducted to compare the sequences of various C. irritans i-antigens isolated in Malaysia with i-antigen sequences of previously reported C. irritans isolates. Phylogenetic comparison was performed to predict the protein's potential to be developed as a screening marker and vaccine candidate against various C. irritans isolates. Multiple sequence alignment of i-antigen amino acid sequences was undertaken using ClustalX and phylogenetic analysis of i-antigen was performed using the maximum parsimony (MP) and Bayesian methods. Nine C. irritans unique transcripts (UTs) had significant matches with i-antigen sequences in the NCBI protein database, with similarities ranging between 41 and 71%. Both MP and Bayesian trees generated showed that i-antigens cn56 and cn57 variants clustered together in one group while other i-antigen variants were divided over two different groups and the grouping was supported by the presence of conserved amino acids in their respective groups. Further research should identify variants of the i-antigen suitable for serodiagnostic candidates and provision of cross-protection against multiple strains of C irritans globally.

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