Phylogenetic analysis of respiratory syncytial virus identified at universiti kebangsaan Malaysia medical centre

Research output: Contribution to journalArticle

Abstract

Human respiratory syncytial virus (RSV) is an important cause of acute respiratory tract infection in infants and young children. Phylogenetic analysis for RSV in Malaysia has not been reported before. We investigated the genetic features of RSV in respiratory specimens from March to August 2011 with molecular methods. From a total of 130 throat swab and nasopharyngeal aspirate specimens, 54 (41.5%) were positive with RSV, identified by in-house real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assay. Thirty-four out of 54 (63.0%) RSV positive patients were children below two years old and two (1.4%) were adults. Phylogenetic analysis showed 39 isolates were genotype GA5, 13 genotypes GA2, one genotype GA1 and one genotype GA7. The findings indicated four genotypes of RSV circulating in the country and the predominant genotype is GA5.

Original languageEnglish
Pages (from-to)739-744
Number of pages6
JournalSains Malaysiana
Volume43
Issue number5
Publication statusPublished - 2014

Fingerprint

Respiratory Syncytial Viruses
Malaysia
Genotype
Human respiratory syncytial virus
Pharynx
Reverse Transcriptase Polymerase Chain Reaction
Respiratory Tract Infections
Real-Time Polymerase Chain Reaction

Keywords

  • GA2
  • GA5 and GA7
  • Genotype GA1
  • Phylogenetic analysis
  • Respiratory syncytial virus

ASJC Scopus subject areas

  • General

Cite this

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title = "Phylogenetic analysis of respiratory syncytial virus identified at universiti kebangsaan Malaysia medical centre",
abstract = "Human respiratory syncytial virus (RSV) is an important cause of acute respiratory tract infection in infants and young children. Phylogenetic analysis for RSV in Malaysia has not been reported before. We investigated the genetic features of RSV in respiratory specimens from March to August 2011 with molecular methods. From a total of 130 throat swab and nasopharyngeal aspirate specimens, 54 (41.5{\%}) were positive with RSV, identified by in-house real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assay. Thirty-four out of 54 (63.0{\%}) RSV positive patients were children below two years old and two (1.4{\%}) were adults. Phylogenetic analysis showed 39 isolates were genotype GA5, 13 genotypes GA2, one genotype GA1 and one genotype GA7. The findings indicated four genotypes of RSV circulating in the country and the predominant genotype is GA5.",
keywords = "GA2, GA5 and GA7, Genotype GA1, Phylogenetic analysis, Respiratory syncytial virus",
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AU - Rahman, Md. Mostafizur

AU - Kon Ken, Wong

AU - Isahak, I.

AU - Zainol Rashid, Noor Zetti

AU - Hanafiah, Alfizah

PY - 2014

Y1 - 2014

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AB - Human respiratory syncytial virus (RSV) is an important cause of acute respiratory tract infection in infants and young children. Phylogenetic analysis for RSV in Malaysia has not been reported before. We investigated the genetic features of RSV in respiratory specimens from March to August 2011 with molecular methods. From a total of 130 throat swab and nasopharyngeal aspirate specimens, 54 (41.5%) were positive with RSV, identified by in-house real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assay. Thirty-four out of 54 (63.0%) RSV positive patients were children below two years old and two (1.4%) were adults. Phylogenetic analysis showed 39 isolates were genotype GA5, 13 genotypes GA2, one genotype GA1 and one genotype GA7. The findings indicated four genotypes of RSV circulating in the country and the predominant genotype is GA5.

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KW - GA5 and GA7

KW - Genotype GA1

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