Photocurable ferrocene-containing poly(2-hydroxyl ethyl methacrylate) films for mediated amperometric glucose biosensor

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28 Citations (Scopus)

Abstract

Ferrocene and its derivatives have been widely used as mediators in biosensor and it is of interest to produce ferrocene-containing (or its derivatives) polymeric films for biosensor application and to prevent leaching of the mediator. In this paper, we report photocurable polymer films for the physical immobilization of ferrocene and vinylferrocene in a low hydrophilicity methacrylate polymer. The hydrophobic-hydrophilic properties of the polymers and its effect on the leaching of the entrapped ferrocene and enzyme were studied. The entrapped ferrocene compounds showed good redox behaviour in these photocured films and the films were used to fabricate a biosensor for the detection of glucose, where the enzyme glucose oxidase (GOD) was incorporated together with the mediator during the photocuring step. Very little or no leaching of the entrapped ferrocene and glucose oxidase was observed. Upon immobilization of the enzyme, the biosensor demonstrated rapid response towards glucose at low potential, yielding a linear glucose response range of 4-17 and 5-42 mM, respectively, for ferrocene and vinylferrocene films. Both glucose biosensors gave reproducibility of <10% relative standard deviations (n=6). The simple and single-step fabrication of the biosensor film is a major advantage when compared to other ferrocene-containing glucose biosensor films.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalThin Solid Films
Volume477
Issue number1-2
DOIs
Publication statusPublished - 22 Apr 2005

Fingerprint

Methacrylates
bioinstrumentation
Biosensors
glucose
Hydroxyl Radical
Glucose
leaching
Leaching
enzymes
Glucose oxidase
Enzymes
oxidase
Glucose Oxidase
immobilization
Polymer films
polymers
Polymers
Derivatives
polymeric films
Hydrophilicity

Keywords

  • Amperometric
  • Ferrocene
  • Mediated biosensor
  • Methacrylate polymers
  • Photocurable

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Condensed Matter Physics
  • Surfaces and Interfaces

Cite this

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title = "Photocurable ferrocene-containing poly(2-hydroxyl ethyl methacrylate) films for mediated amperometric glucose biosensor",
abstract = "Ferrocene and its derivatives have been widely used as mediators in biosensor and it is of interest to produce ferrocene-containing (or its derivatives) polymeric films for biosensor application and to prevent leaching of the mediator. In this paper, we report photocurable polymer films for the physical immobilization of ferrocene and vinylferrocene in a low hydrophilicity methacrylate polymer. The hydrophobic-hydrophilic properties of the polymers and its effect on the leaching of the entrapped ferrocene and enzyme were studied. The entrapped ferrocene compounds showed good redox behaviour in these photocured films and the films were used to fabricate a biosensor for the detection of glucose, where the enzyme glucose oxidase (GOD) was incorporated together with the mediator during the photocuring step. Very little or no leaching of the entrapped ferrocene and glucose oxidase was observed. Upon immobilization of the enzyme, the biosensor demonstrated rapid response towards glucose at low potential, yielding a linear glucose response range of 4-17 and 5-42 mM, respectively, for ferrocene and vinylferrocene films. Both glucose biosensors gave reproducibility of <10{\%} relative standard deviations (n=6). The simple and single-step fabrication of the biosensor film is a major advantage when compared to other ferrocene-containing glucose biosensor films.",
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author = "Bean, {Low Sim} and Lee, {Yook Heng} and {Mohd. Yamin}, Bohari and Musa Ahmad",
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AU - Ahmad, Musa

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AB - Ferrocene and its derivatives have been widely used as mediators in biosensor and it is of interest to produce ferrocene-containing (or its derivatives) polymeric films for biosensor application and to prevent leaching of the mediator. In this paper, we report photocurable polymer films for the physical immobilization of ferrocene and vinylferrocene in a low hydrophilicity methacrylate polymer. The hydrophobic-hydrophilic properties of the polymers and its effect on the leaching of the entrapped ferrocene and enzyme were studied. The entrapped ferrocene compounds showed good redox behaviour in these photocured films and the films were used to fabricate a biosensor for the detection of glucose, where the enzyme glucose oxidase (GOD) was incorporated together with the mediator during the photocuring step. Very little or no leaching of the entrapped ferrocene and glucose oxidase was observed. Upon immobilization of the enzyme, the biosensor demonstrated rapid response towards glucose at low potential, yielding a linear glucose response range of 4-17 and 5-42 mM, respectively, for ferrocene and vinylferrocene films. Both glucose biosensors gave reproducibility of <10% relative standard deviations (n=6). The simple and single-step fabrication of the biosensor film is a major advantage when compared to other ferrocene-containing glucose biosensor films.

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