Phenotypic expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes.

A. N. Nur Adelina, B. S. Aminuddin, S. Munirah, Chua Kien Hui, N. H. Fuzina, L. Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cartilage is regularly needed for reconstructive surgery. Basic research in tissue engineering is necessary to develop its full potential. We presented here the expression profile of type II collagen gene and type I collagen gene in human auricular monolayer culture expansion. Cultured chondrocytes documented a reduction in the expression level of collagen type II gene whilst collagen type I gene was gradually expressed through all the passages. This study demonstrated that human auricular chondrocytes lose its phenotypic expression during monolayer culture expansion. Further studies are required to enhance cartilage specific gene expression, collagen type II throughout the in vitro culture.

Original languageEnglish
Pages (from-to)188-189
Number of pages2
JournalThe Medical journal of Malaysia
Volume59 Suppl B
Publication statusPublished - May 2004

Fingerprint

Collagen Type II
Chondrocytes
Collagen Type I
Genes
Cartilage
Reconstructive Surgical Procedures
Tissue Engineering
Collagen
Gene Expression
Research

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Phenotypic expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes. / Nur Adelina, A. N.; Aminuddin, B. S.; Munirah, S.; Kien Hui, Chua; Fuzina, N. H.; Saim, L.; Idrus, Ruszymah.

In: The Medical journal of Malaysia, Vol. 59 Suppl B, 05.2004, p. 188-189.

Research output: Contribution to journalArticle

Nur Adelina, A. N. ; Aminuddin, B. S. ; Munirah, S. ; Kien Hui, Chua ; Fuzina, N. H. ; Saim, L. ; Idrus, Ruszymah. / Phenotypic expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes. In: The Medical journal of Malaysia. 2004 ; Vol. 59 Suppl B. pp. 188-189.
@article{0db5aaccabb746999161a1db73842065,
title = "Phenotypic expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes.",
abstract = "Cartilage is regularly needed for reconstructive surgery. Basic research in tissue engineering is necessary to develop its full potential. We presented here the expression profile of type II collagen gene and type I collagen gene in human auricular monolayer culture expansion. Cultured chondrocytes documented a reduction in the expression level of collagen type II gene whilst collagen type I gene was gradually expressed through all the passages. This study demonstrated that human auricular chondrocytes lose its phenotypic expression during monolayer culture expansion. Further studies are required to enhance cartilage specific gene expression, collagen type II throughout the in vitro culture.",
author = "{Nur Adelina}, {A. N.} and Aminuddin, {B. S.} and S. Munirah and {Kien Hui}, Chua and Fuzina, {N. H.} and L. Saim and Ruszymah Idrus",
year = "2004",
month = "5",
language = "English",
volume = "59 Suppl B",
pages = "188--189",
journal = "Medical Journal of Malaysia",
issn = "0300-5283",
publisher = "Malaysian Medical Association",

}

TY - JOUR

T1 - Phenotypic expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes.

AU - Nur Adelina, A. N.

AU - Aminuddin, B. S.

AU - Munirah, S.

AU - Kien Hui, Chua

AU - Fuzina, N. H.

AU - Saim, L.

AU - Idrus, Ruszymah

PY - 2004/5

Y1 - 2004/5

N2 - Cartilage is regularly needed for reconstructive surgery. Basic research in tissue engineering is necessary to develop its full potential. We presented here the expression profile of type II collagen gene and type I collagen gene in human auricular monolayer culture expansion. Cultured chondrocytes documented a reduction in the expression level of collagen type II gene whilst collagen type I gene was gradually expressed through all the passages. This study demonstrated that human auricular chondrocytes lose its phenotypic expression during monolayer culture expansion. Further studies are required to enhance cartilage specific gene expression, collagen type II throughout the in vitro culture.

AB - Cartilage is regularly needed for reconstructive surgery. Basic research in tissue engineering is necessary to develop its full potential. We presented here the expression profile of type II collagen gene and type I collagen gene in human auricular monolayer culture expansion. Cultured chondrocytes documented a reduction in the expression level of collagen type II gene whilst collagen type I gene was gradually expressed through all the passages. This study demonstrated that human auricular chondrocytes lose its phenotypic expression during monolayer culture expansion. Further studies are required to enhance cartilage specific gene expression, collagen type II throughout the in vitro culture.

UR - http://www.scopus.com/inward/record.url?scp=16544391389&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16544391389&partnerID=8YFLogxK

M3 - Article

VL - 59 Suppl B

SP - 188

EP - 189

JO - Medical Journal of Malaysia

JF - Medical Journal of Malaysia

SN - 0300-5283

ER -