Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells

Kar Wey Yong, Belinda Pingguan-Murphy, Feng Xu, Wan Abu Bakar Wan Abas, Jane Ru Choi, Siti Zawiah Omar, Mat Adenan Noor Azmi, Chua Kien Hui, Wan Kamarul Zaman Wan Safwani

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50 Citations (Scopus)

Abstract

Cryopreservation represents an effective technique to maintain the functional properties of human adipose-derived stem cells (ASCs) and allows pooling of cells via long-term storage for clinical applications, e.g., cell-based therapies. It is crucial to reduce freezing injury during the cryopreservation process by loading the ASCs with the optimum concentration of suitable cryoprotective agents (CPAs). In this study, human ASCs were preserved for 3 months in different combinations of CPAs, including 1) 0.25 M trehalose; 2) 5% dimethylsulfoxide (DMSO); 3) 10% DMSO; 4) 5% DMSO + 20% fetal bovine serum (FBS); 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS. Interestingly, even with a reduction of DMSO to 5% and without FBS, cryopreserved ASCs maintained high cell viability comparable with standard cryomedium (10% DMSO + 90% FBS), with normal cell phenotype and proliferation rate. Cryopreserved ASCs also maintained their differentiation capability (e.g., to adipocytes, osteocytes and chondrocytes) and showed an enhanced expression level of stemness markers (e.g., NANOG, OCT-4, SOX-2 and REX-1). Our findings suggest that 5% DMSO without FBS may be an ideal CPA for an efficient long-term cryopreservation of human ASCs. These results aid in establishing standardized xeno-free long-term cryopreservation of human ASCs for clinical applications.

Original languageEnglish
Article number9596
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 15 Apr 2015

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Dimethyl Sulfoxide
Stem Cells
Cryoprotective Agents
Cryopreservation
Serum
Osteocytes
Trehalose
Chondrocytes
Cell- and Tissue-Based Therapy
Adipocytes
Freezing
Cell Survival
Cell Proliferation
Phenotype
Wounds and Injuries

ASJC Scopus subject areas

  • General

Cite this

Yong, K. W., Pingguan-Murphy, B., Xu, F., Abas, W. A. B. W., Choi, J. R., Omar, S. Z., ... Safwani, W. K. Z. W. (2015). Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells. Scientific Reports, 5, [9596]. https://doi.org/10.1038/srep09596

Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells. / Yong, Kar Wey; Pingguan-Murphy, Belinda; Xu, Feng; Abas, Wan Abu Bakar Wan; Choi, Jane Ru; Omar, Siti Zawiah; Azmi, Mat Adenan Noor; Kien Hui, Chua; Safwani, Wan Kamarul Zaman Wan.

In: Scientific Reports, Vol. 5, 9596, 15.04.2015.

Research output: Contribution to journalArticle

Yong, KW, Pingguan-Murphy, B, Xu, F, Abas, WABW, Choi, JR, Omar, SZ, Azmi, MAN, Kien Hui, C & Safwani, WKZW 2015, 'Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells', Scientific Reports, vol. 5, 9596. https://doi.org/10.1038/srep09596
Yong, Kar Wey ; Pingguan-Murphy, Belinda ; Xu, Feng ; Abas, Wan Abu Bakar Wan ; Choi, Jane Ru ; Omar, Siti Zawiah ; Azmi, Mat Adenan Noor ; Kien Hui, Chua ; Safwani, Wan Kamarul Zaman Wan. / Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells. In: Scientific Reports. 2015 ; Vol. 5.
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