Phenol biodegradation and metal removal by a mixed bacterial consortium

Kok Kee Wong, Brid Quilty, Ainon Hamzah, Salmijah Surif

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    This paper reports the tolerance and biodegradation of phenol by a heavy metal-adapted environmental bacterial consortium, known as consortium culture (CC). At the highest tolerable phenol concentration of 1200 mg/L, CC displayed specific growth rate of 0.04 h<sup>-1</sup>, phenol degradation rate of 6.11 mg L<sup>-1</sup> h<sup>-1</sup> and biomass of 8.45 ± 0.35 (log10 colony-forming units [CFU]/ml) at the end of incubation. Phenol was degraded via the ortho-cleavage pathway catalyzed by cathechol-1,2-dioxygenase with specific activity of 0.083 (mol min<sup>-1</sup> mg<sup>-1</sup> protein). The different constituent bacterial isolates of CC preferentially grow on benzene, toluene, xylene, ethylbenzene, cresol, and catechol, suggesting a synergistic mechanism involved in the degradation process. Microtox assay showed that phenol degradation was achieved without producing toxic dead-end metabolites. Moreover, lead (Pb) and cadmium (Cd) at the highest tested concentration of 1.0 and 0.1 mg/L, respectively, did not inhibit phenol degradation by CC. Simultaneous metal removal during phenol degradation was achieved using CC. These findings confirmed the dual function of CC to degrade phenol and to remove heavy metals from a mixed-pollutant medium.

    Original languageEnglish
    Pages (from-to)104-112
    Number of pages9
    JournalBioremediation Journal
    Volume19
    Issue number2
    DOIs
    Publication statusPublished - 3 Apr 2015

    Fingerprint

    phenol
    biodegradation
    metal
    degradation
    heavy metal
    BTEX
    removal
    cleavage
    metabolite
    cadmium
    tolerance
    incubation
    assay
    protein
    pollutant
    biomass

    Keywords

    • biodegradation
    • cadmium
    • consortium culture
    • lead
    • phenol

    ASJC Scopus subject areas

    • Environmental Science(all)

    Cite this

    Phenol biodegradation and metal removal by a mixed bacterial consortium. / Wong, Kok Kee; Quilty, Brid; Hamzah, Ainon; Surif, Salmijah.

    In: Bioremediation Journal, Vol. 19, No. 2, 03.04.2015, p. 104-112.

    Research output: Contribution to journalArticle

    Wong, Kok Kee ; Quilty, Brid ; Hamzah, Ainon ; Surif, Salmijah. / Phenol biodegradation and metal removal by a mixed bacterial consortium. In: Bioremediation Journal. 2015 ; Vol. 19, No. 2. pp. 104-112.
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