Phage displayed Bacillus thuringiensis Cry1Ba4 toxin is toxic to Plutella xylostella

Sheila Nathan, Do'a Hamzah A Aziz, Nor M. Mahadi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We constructed recombinant phage particles displaying the Bacillus thuringiensis Cry1Ba4 active toxin using the pfUSE5 and pComb3X phagemid vectors. The recombinant phage particles were screened and evaluated for displayed biologically active Cry1Ba4 toxin against the target insect larvae. Concurrent expression of Cry1Ba4 protoxin was carried out using the pETBlue™-2 plasmid expression vector in Escherichia coli Tuner™(DE3)pLacI and the protoxin was successfully expressed at a size of 129 kDa. In the bioassay, 3.30 mg crude extract of Cry1Ba4 protoxin, 9.35 × 109 TU and 7.70 × 109 TU of induced recombinant phage particles carrying Cry1Ba4 active toxin displayed on pComb3X and pFUSE5, respectively, demonstrated mortality of greater than 85% against Plutella xylostella (third-instar) within 48 hours. Thus, we have successfully displayed the Cry1Ba4 activated toxin on the surface of a phage and demonstrated toxicity towards larvae.

Original languageEnglish
Pages (from-to)412-415
Number of pages4
JournalCurrent Microbiology
Volume53
Issue number5
DOIs
Publication statusPublished - Nov 2006

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Bacillus thuringiensis
Poisons
Bacteriophages
Larva
Complex Mixtures
Biological Assay
Insects
Plasmids
Escherichia coli
Mortality

ASJC Scopus subject areas

  • Microbiology

Cite this

Phage displayed Bacillus thuringiensis Cry1Ba4 toxin is toxic to Plutella xylostella. / Nathan, Sheila; Aziz, Do'a Hamzah A; Mahadi, Nor M.

In: Current Microbiology, Vol. 53, No. 5, 11.2006, p. 412-415.

Research output: Contribution to journalArticle

Nathan, Sheila ; Aziz, Do'a Hamzah A ; Mahadi, Nor M. / Phage displayed Bacillus thuringiensis Cry1Ba4 toxin is toxic to Plutella xylostella. In: Current Microbiology. 2006 ; Vol. 53, No. 5. pp. 412-415.
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