Perturbation of cellular immune functions in cigarette smokers and protection by palm oil vitamin e supplementation

Zakiah Jubri, Azian Abdul Latif, Abdul Gapor Md Top, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Cigarette smoke contains free radicals and an have adverse effect to the immune system. Supplementation of palm oil vitamin E (palmvitee), is known has antioxidant properties is thought to be beneficial for system immune protection against free radicals activity. The objective of the study was to determine the effect of palmvitee supplementation on immune response in smokers. Methods. This study involved a group of smokers and nonsmokers who received 200 mg/day palmvitee and placebo for the control group. Blood samples were taken at 0, 12 and 24 weeks of supplementation. Plasma tocopherol and tocotrienol were determined by HPLC, lymphocyte proliferation by lymphocyte transformation test (LTT) and enumeration of lymphocytes T and B cells by flow cytometry. Statistical analysis was performed by Mann-Whitney U-test for non-parametric data distribution and correlation among the variables was examined by Spearman. Results: Plasma tocopherol and tocotrienol were increased in vitamin E supplemented group as compared to placebo group. Urine cotinine levels and serum §ssub§1§esub§-antitrypsin were significantly higher in smokers compared to nonsmokers. Lymphocyte proliferation induced by PHA showed an increasing trend with palmvitee supplementation in both smokers and nonsmokers. Natural killer cells were decreased; CD4§ssup§+§esup§ cells and B cells were increased in smokers compared to nonsmokers but were unaffected with vitamin E supplementation except in the percentage of B cells which were increased in nonsmokers supplemented palmvitee compared to placebo. CD4§ssup§+§esup§ /CD8§ssup§+§esup§ ratio was increased in smokers compared to nonsmokers. The high TWBC count observed in smokers correlated with the increased CD4§ssup§+§esup§ and B cells. Conclusions: Smoking caused alterations in certain immune parameters and palmvitee supplementation tended to cause an increase in lymphocytes transformation test but had no effect on CD3§ssup§+§esup§, CD4§ssup§+§esup§, CD8§ssup§+§esup§, NK cells and B cells except B cells percentage in nonsmokers.

Original languageEnglish
Article number2
JournalNutrition Journal
Volume12
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Vitamins
Tobacco Products
B-Lymphocytes
Vitamin E
Tocotrienols
Tocopherols
Placebos
Lymphocytes
Lymphocyte Activation
Natural Killer Cells
Free Radicals
Immune System
Cotinine
Nonparametric Statistics
Smoke
palm oil
Flow Cytometry
Antioxidants
Smoking
High Pressure Liquid Chromatography

Keywords

  • Cellular immune functions
  • Cigarette smoke
  • Tocopherol
  • Tocotrienol

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Perturbation of cellular immune functions in cigarette smokers and protection by palm oil vitamin e supplementation. / Jubri, Zakiah; Latif, Azian Abdul; Top, Abdul Gapor Md; Ngah, Wan Zurinah Wan.

In: Nutrition Journal, Vol. 12, No. 1, 2, 2013.

Research output: Contribution to journalArticle

Jubri, Zakiah ; Latif, Azian Abdul ; Top, Abdul Gapor Md ; Ngah, Wan Zurinah Wan. / Perturbation of cellular immune functions in cigarette smokers and protection by palm oil vitamin e supplementation. In: Nutrition Journal. 2013 ; Vol. 12, No. 1.
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AU - Latif, Azian Abdul

AU - Top, Abdul Gapor Md

AU - Ngah, Wan Zurinah Wan

PY - 2013

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N2 - Background: Cigarette smoke contains free radicals and an have adverse effect to the immune system. Supplementation of palm oil vitamin E (palmvitee), is known has antioxidant properties is thought to be beneficial for system immune protection against free radicals activity. The objective of the study was to determine the effect of palmvitee supplementation on immune response in smokers. Methods. This study involved a group of smokers and nonsmokers who received 200 mg/day palmvitee and placebo for the control group. Blood samples were taken at 0, 12 and 24 weeks of supplementation. Plasma tocopherol and tocotrienol were determined by HPLC, lymphocyte proliferation by lymphocyte transformation test (LTT) and enumeration of lymphocytes T and B cells by flow cytometry. Statistical analysis was performed by Mann-Whitney U-test for non-parametric data distribution and correlation among the variables was examined by Spearman. Results: Plasma tocopherol and tocotrienol were increased in vitamin E supplemented group as compared to placebo group. Urine cotinine levels and serum §ssub§1§esub§-antitrypsin were significantly higher in smokers compared to nonsmokers. Lymphocyte proliferation induced by PHA showed an increasing trend with palmvitee supplementation in both smokers and nonsmokers. Natural killer cells were decreased; CD4§ssup§+§esup§ cells and B cells were increased in smokers compared to nonsmokers but were unaffected with vitamin E supplementation except in the percentage of B cells which were increased in nonsmokers supplemented palmvitee compared to placebo. CD4§ssup§+§esup§ /CD8§ssup§+§esup§ ratio was increased in smokers compared to nonsmokers. The high TWBC count observed in smokers correlated with the increased CD4§ssup§+§esup§ and B cells. Conclusions: Smoking caused alterations in certain immune parameters and palmvitee supplementation tended to cause an increase in lymphocytes transformation test but had no effect on CD3§ssup§+§esup§, CD4§ssup§+§esup§, CD8§ssup§+§esup§, NK cells and B cells except B cells percentage in nonsmokers.

AB - Background: Cigarette smoke contains free radicals and an have adverse effect to the immune system. Supplementation of palm oil vitamin E (palmvitee), is known has antioxidant properties is thought to be beneficial for system immune protection against free radicals activity. The objective of the study was to determine the effect of palmvitee supplementation on immune response in smokers. Methods. This study involved a group of smokers and nonsmokers who received 200 mg/day palmvitee and placebo for the control group. Blood samples were taken at 0, 12 and 24 weeks of supplementation. Plasma tocopherol and tocotrienol were determined by HPLC, lymphocyte proliferation by lymphocyte transformation test (LTT) and enumeration of lymphocytes T and B cells by flow cytometry. Statistical analysis was performed by Mann-Whitney U-test for non-parametric data distribution and correlation among the variables was examined by Spearman. Results: Plasma tocopherol and tocotrienol were increased in vitamin E supplemented group as compared to placebo group. Urine cotinine levels and serum §ssub§1§esub§-antitrypsin were significantly higher in smokers compared to nonsmokers. Lymphocyte proliferation induced by PHA showed an increasing trend with palmvitee supplementation in both smokers and nonsmokers. Natural killer cells were decreased; CD4§ssup§+§esup§ cells and B cells were increased in smokers compared to nonsmokers but were unaffected with vitamin E supplementation except in the percentage of B cells which were increased in nonsmokers supplemented palmvitee compared to placebo. CD4§ssup§+§esup§ /CD8§ssup§+§esup§ ratio was increased in smokers compared to nonsmokers. The high TWBC count observed in smokers correlated with the increased CD4§ssup§+§esup§ and B cells. Conclusions: Smoking caused alterations in certain immune parameters and palmvitee supplementation tended to cause an increase in lymphocytes transformation test but had no effect on CD3§ssup§+§esup§, CD4§ssup§+§esup§, CD8§ssup§+§esup§, NK cells and B cells except B cells percentage in nonsmokers.

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KW - Cigarette smoke

KW - Tocopherol

KW - Tocotrienol

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