Perigone Lobe Transcriptome Analysis Provides Insights into Rafflesia cantleyi Flower Development

Xin Wei Lee, Mohd Noor Mat-Isa, Nur Atiqah Mohd-Elias, Mohd Afiq Aizat-Juhari, Hoe Han Goh, Paul H. Dear, Keng See Chow, Jumaat Adam, Rahmah Mohamed, Mohd Firdaus Mohd Raih, Kiew Lian Wan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rafflesia is a biologically enigmatic species that is very rare in occurrence and possesses an extraordinary morphology. This parasitic plant produces a gigantic flower up to one metre in diameter with no leaves, stem or roots. However, little is known about the floral biology of this species especially at the molecular level. In an effort to address this issue, we have generated and characterised the transcriptome of the Rafflesia cantleyi flower, and performed a comparison with the transcriptome of its floral bud to predict genes that are expressed and regulated during flower development. Approximately 40 million sequencing reads were generated and assembled de novo into 18,053 transcripts with an average length of 641 bp. Of these, more than 79% of the transcripts had significant matches to annotated sequences in the public protein database. A total of 11,756 and 7,891 transcripts were assigned to Gene Ontology categories and clusters of orthologous groups respectively. In addition, 6,019 transcripts could be mapped to 129 pathways in Kyoto Encyclopaedia of Genes and Genomes Pathway database. Digital abundance analysis identified 52 transcripts with very high expression in the flower transcriptome of R. cantleyi. Subsequently, analysis of differential expression between developing flower and the floral bud revealed a set of 105 transcripts with potential role in flower development. Our work presents a deep transcriptome resource analysis for the developing flower of R. cantleyi. Genes potentially involved in the growth and development of the R. cantleyi flower were identified and provide insights into biological processes that occur during flower development.

Original languageEnglish
Article numbere0167958
JournalPLoS One
Volume11
Issue number12
DOIs
Publication statusPublished - 1 Dec 2016

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Rafflesia
Gene Expression Profiling
transcriptomics
Genes
flowering
flowers
transcriptome
Transcriptome
genes
buds
parasitic plants
Ontology
Biological Phenomena
Protein Databases
Gene Ontology
growth and development
Multigene Family
Growth and Development
Biological Sciences
stems

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Perigone Lobe Transcriptome Analysis Provides Insights into Rafflesia cantleyi Flower Development. / Lee, Xin Wei; Mat-Isa, Mohd Noor; Mohd-Elias, Nur Atiqah; Aizat-Juhari, Mohd Afiq; Goh, Hoe Han; Dear, Paul H.; Chow, Keng See; Adam, Jumaat; Mohamed, Rahmah; Mohd Raih, Mohd Firdaus; Wan, Kiew Lian.

In: PLoS One, Vol. 11, No. 12, e0167958, 01.12.2016.

Research output: Contribution to journalArticle

Lee, XW, Mat-Isa, MN, Mohd-Elias, NA, Aizat-Juhari, MA, Goh, HH, Dear, PH, Chow, KS, Adam, J, Mohamed, R, Mohd Raih, MF & Wan, KL 2016, 'Perigone Lobe Transcriptome Analysis Provides Insights into Rafflesia cantleyi Flower Development', PLoS One, vol. 11, no. 12, e0167958. https://doi.org/10.1371/journal.pone.0167958
Lee, Xin Wei ; Mat-Isa, Mohd Noor ; Mohd-Elias, Nur Atiqah ; Aizat-Juhari, Mohd Afiq ; Goh, Hoe Han ; Dear, Paul H. ; Chow, Keng See ; Adam, Jumaat ; Mohamed, Rahmah ; Mohd Raih, Mohd Firdaus ; Wan, Kiew Lian. / Perigone Lobe Transcriptome Analysis Provides Insights into Rafflesia cantleyi Flower Development. In: PLoS One. 2016 ; Vol. 11, No. 12.
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