Pengecaman awal komuniti bakteria sel bahan api mikrob dalam air sisa kumbahan

Research output: Contribution to journalArticle

Abstract

This study aimed to determine the types of bacteria exist in wastewater that contibute to generate electricity and simultaneously remove carbon and nitrogen. The method used was Fluorescence In Situ Hibridization (FISH) to detect the bacteria group while Polymerase Chain Reaction (PCR) was used to confirm the observation made using FISH. A biochemical identification using BIOLOG GEN III MICROPLATE™ also was used . The samples were cultured on nutrient agar plate to identify the morphology of the bacteria. The result showed that 21 isolates from three different locations at the activated sludge treatment plant with six, eleven and four strains for raw sewage, aeration tank and returned activated sludge samples, respectively. Preliminary identification does not give a good match but only showed the existence of ammonia-oxidizing bacteria (FISH) and Kurtia Gibsoni (BIOLOG) from aeration tank : Bacillus sp (PCR) and Bacillus Pseudomycoides (BIOLOG) from returned activated sludge. The maximum power density generated using returned activated sludge was 9.053 mW/cm2, with 26.8% COD removal and 40% TKN removal.

Original languageEnglish
Pages (from-to)959-964
Number of pages6
JournalSains Malaysiana
Volume40
Issue number9
Publication statusPublished - Sep 2011

Fingerprint

activated sludge
bacterium
fluorescence
air
polymerase chain reaction
aeration
agar
electricity
sewage
ammonia
wastewater
nutrient
nitrogen
carbon
in situ
removal

Keywords

  • Bacteria
  • Fluorescence in situ hibridization(FISH)
  • Microbial fuel cell
  • Polymerasa chain reaction(PCR)
  • Sewage

ASJC Scopus subject areas

  • General

Cite this

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title = "Pengecaman awal komuniti bakteria sel bahan api mikrob dalam air sisa kumbahan",
abstract = "This study aimed to determine the types of bacteria exist in wastewater that contibute to generate electricity and simultaneously remove carbon and nitrogen. The method used was Fluorescence In Situ Hibridization (FISH) to detect the bacteria group while Polymerase Chain Reaction (PCR) was used to confirm the observation made using FISH. A biochemical identification using BIOLOG GEN III MICROPLATE™ also was used . The samples were cultured on nutrient agar plate to identify the morphology of the bacteria. The result showed that 21 isolates from three different locations at the activated sludge treatment plant with six, eleven and four strains for raw sewage, aeration tank and returned activated sludge samples, respectively. Preliminary identification does not give a good match but only showed the existence of ammonia-oxidizing bacteria (FISH) and Kurtia Gibsoni (BIOLOG) from aeration tank : Bacillus sp (PCR) and Bacillus Pseudomycoides (BIOLOG) from returned activated sludge. The maximum power density generated using returned activated sludge was 9.053 mW/cm2, with 26.8{\%} COD removal and 40{\%} TKN removal.",
keywords = "Bacteria, Fluorescence in situ hibridization(FISH), Microbial fuel cell, Polymerasa chain reaction(PCR), Sewage",
author = "{Md Zain}, Shahrom and Roosfa Hashim and Roslani, {N. S.} and Nurina Anuar and Fatihah Suja` and {Wan Daud}, {Wan Ramli} and {Ahmad Basri}, {Noor Ezlin}",
year = "2011",
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AU - Md Zain, Shahrom

AU - Hashim, Roosfa

AU - Roslani, N. S.

AU - Anuar, Nurina

AU - Suja`, Fatihah

AU - Wan Daud, Wan Ramli

AU - Ahmad Basri, Noor Ezlin

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N2 - This study aimed to determine the types of bacteria exist in wastewater that contibute to generate electricity and simultaneously remove carbon and nitrogen. The method used was Fluorescence In Situ Hibridization (FISH) to detect the bacteria group while Polymerase Chain Reaction (PCR) was used to confirm the observation made using FISH. A biochemical identification using BIOLOG GEN III MICROPLATE™ also was used . The samples were cultured on nutrient agar plate to identify the morphology of the bacteria. The result showed that 21 isolates from three different locations at the activated sludge treatment plant with six, eleven and four strains for raw sewage, aeration tank and returned activated sludge samples, respectively. Preliminary identification does not give a good match but only showed the existence of ammonia-oxidizing bacteria (FISH) and Kurtia Gibsoni (BIOLOG) from aeration tank : Bacillus sp (PCR) and Bacillus Pseudomycoides (BIOLOG) from returned activated sludge. The maximum power density generated using returned activated sludge was 9.053 mW/cm2, with 26.8% COD removal and 40% TKN removal.

AB - This study aimed to determine the types of bacteria exist in wastewater that contibute to generate electricity and simultaneously remove carbon and nitrogen. The method used was Fluorescence In Situ Hibridization (FISH) to detect the bacteria group while Polymerase Chain Reaction (PCR) was used to confirm the observation made using FISH. A biochemical identification using BIOLOG GEN III MICROPLATE™ also was used . The samples were cultured on nutrient agar plate to identify the morphology of the bacteria. The result showed that 21 isolates from three different locations at the activated sludge treatment plant with six, eleven and four strains for raw sewage, aeration tank and returned activated sludge samples, respectively. Preliminary identification does not give a good match but only showed the existence of ammonia-oxidizing bacteria (FISH) and Kurtia Gibsoni (BIOLOG) from aeration tank : Bacillus sp (PCR) and Bacillus Pseudomycoides (BIOLOG) from returned activated sludge. The maximum power density generated using returned activated sludge was 9.053 mW/cm2, with 26.8% COD removal and 40% TKN removal.

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