Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage

Ruszymah Idrus, B. S. Lokman, Asma Abdullah, S. Munirah, Chua Kien Hui, Mazlyzam Abdul Latif, M. R. Isa, N. H. Fuzina, B. S. Aminuddin

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objectives: This study was aimed at regenerating autologous elastic cartilage for future use in pediatric ear reconstruction surgery. Specific attentions were to characterize pediatric auricular chondrocyte growth in a combination culture medium and to assess the possibility of elastic cartilage regeneration using human fibrin. Study design: Laboratory experiment using human pediatric auricular chondrocytes. Methods: Pediatric auricular chondrocytes growth kinetics and quantitative gene expression profile in three different types of media were compared in primary culture and subsequent three passages. Large-scale culture-expanded chondrocytes from the combination medium were then mixed with human fibrin for the formation of elastic cartilage via tissue engineering technique. Results: The equal mixture of Ham's F12 and Dulbecco's Modified Eagle Medium (FD) promoted the best chondrocyte growth at every passage compared to the individual media. Chondrocytes differentiation index; ratio of type II to type I collagen gene expression level, aggrecan and elastin expression gradually decreased while passaging but they were then restored in engineered tissues after implantation. The engineered cartilage was glistening white in color and firm in consistency. Histological evaluation, immunohistochemistry analysis and quantitative gene expression assessment demonstrated that the engineered cartilage resemble the features of native elastic cartilage. Conclusion: Pediatric auricular chondrocytes proliferate better in the combination medium (FD) and the utilization of human fibrin as a biomaterial hold promises for the regeneration of an autologous elastic cartilage for future application in ear reconstructive surgery.

Original languageEnglish
Pages (from-to)1225-1234
Number of pages10
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume71
Issue number8
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Elastic Cartilage
Chondrocytes
Pediatrics
Gene Expression
Fibrin
Cartilage
Ear
Regeneration
Reconstructive Surgical Procedures
Growth
Aggrecans
Eagles
Elastin
Biocompatible Materials
Tissue Engineering
Collagen Type I
Transcriptome
Culture Media
Color
Immunohistochemistry

Keywords

  • Auricular chondrocyte
  • Elastic cartilage
  • Gene expression analysis
  • Human fibrin
  • Tissue engineering

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Pediatrics, Perinatology, and Child Health
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage. / Idrus, Ruszymah; Lokman, B. S.; Abdullah, Asma; Munirah, S.; Kien Hui, Chua; Abdul Latif, Mazlyzam; Isa, M. R.; Fuzina, N. H.; Aminuddin, B. S.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 71, No. 8, 08.2007, p. 1225-1234.

Research output: Contribution to journalArticle

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