Pathotyping of Newcastle disease virus with a filamentous bacteriophage

Priadarishni Ramanujam, Wen Siang Tan, Sheila Nathan, Khatijah Yusoff

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A filamentous phage bearing the peptide sequence TLTTKLY was isolated from a heptapeptide phage display library against a velogenic Newcastle disease virus (NDV). In order to investigate the potential of this specific phage as an immunological reagent in virus pathotyping, an enzyme-linked immunosorbent assay (ELISA)-based method was developed. This method can differentiate the velogenic strains from the mesogenic and lentogenic strains. An equilibrium-binding assay in solution showed that the interactions between the phage and all the NDV strains gave rise to two widely differing dissociation constants (Kdrel). Based upon the first Kd rel values, NDV strains can be classified into two groups; the first comprises the velogenic strains, and the second consists of the mesogenic and lentogenic strains. These results indicate a high degree of correlation between the binding affinities and pathotyping of NDV strains using the TLTTKLY phage.

Original languageEnglish
Pages (from-to)296-302
Number of pages7
JournalBioTechniques
Volume36
Issue number2
Publication statusPublished - Feb 2004

Fingerprint

Inovirus
Newcastle disease virus
Bacteriophages
Viruses
Assays
Bearings (structural)
Libraries
Immunosorbents
Enzyme-Linked Immunosorbent Assay
Peptides
Display devices

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ramanujam, P., Tan, W. S., Nathan, S., & Yusoff, K. (2004). Pathotyping of Newcastle disease virus with a filamentous bacteriophage. BioTechniques, 36(2), 296-302.

Pathotyping of Newcastle disease virus with a filamentous bacteriophage. / Ramanujam, Priadarishni; Tan, Wen Siang; Nathan, Sheila; Yusoff, Khatijah.

In: BioTechniques, Vol. 36, No. 2, 02.2004, p. 296-302.

Research output: Contribution to journalArticle

Ramanujam, P, Tan, WS, Nathan, S & Yusoff, K 2004, 'Pathotyping of Newcastle disease virus with a filamentous bacteriophage', BioTechniques, vol. 36, no. 2, pp. 296-302.
Ramanujam, Priadarishni ; Tan, Wen Siang ; Nathan, Sheila ; Yusoff, Khatijah. / Pathotyping of Newcastle disease virus with a filamentous bacteriophage. In: BioTechniques. 2004 ; Vol. 36, No. 2. pp. 296-302.
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