Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells

S. M Z H Chowdhury, A. R. Omar, I. Aini, M. Hair-Bejo, A. A. Jamaluddin, Badrul Munir Md. Zain, Y. Kono

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Specific-pathogen-free (SPF) chickens inoculated with low passage Chicken anaemia virus (CAV), SMSC-1 and 3-1 isolates produced lesions suggestive of CAV infection. Repeated passages of the isolates in cell culture until passage 60 (P60) and passage 123 produced viruses that showed a significantly reduced level of pathogenicity in SPF chickens compared to the low passage isolates. Sequence comparison indicated that nucleotide changes in only the coding region of the P60 passage isolates were thought to contribute to virus attenuation. Phylogenetic analysis indicated that SMSC-1 and 3-1 were highly divergent, but their P60 passage derivatives shared significant homology to a Japanese isolate A2.

Original languageEnglish
Pages (from-to)2437-2448
Number of pages12
JournalArchives of Virology
Volume148
Issue number12
DOIs
Publication statusPublished - Dec 2003

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Chicken anemia virus
Specific Pathogen-Free Organisms
Sequence Analysis
Virulence
Chickens
Viruses
Virus Diseases
varespladib methyl
Nucleotides
Cell Culture Techniques

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

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Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells. / Chowdhury, S. M Z H; Omar, A. R.; Aini, I.; Hair-Bejo, M.; Jamaluddin, A. A.; Md. Zain, Badrul Munir; Kono, Y.

In: Archives of Virology, Vol. 148, No. 12, 12.2003, p. 2437-2448.

Research output: Contribution to journalArticle

Chowdhury, S. M Z H ; Omar, A. R. ; Aini, I. ; Hair-Bejo, M. ; Jamaluddin, A. A. ; Md. Zain, Badrul Munir ; Kono, Y. / Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells. In: Archives of Virology. 2003 ; Vol. 148, No. 12. pp. 2437-2448.
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