Paternal high-fat diet consumption induces common changes in the transcriptomes of retroperitoneal adipose and pancreatic islet tissues in female rat offspring

Sheau Fang Ng, Ruby C Y Lin, Christopher A. Maloney, Neil A. Youngson, Julie A. Owens, Margaret J. Morris

    Research output: Contribution to journalArticle

    70 Citations (Scopus)

    Abstract

    We previously showed that paternal high-fat diet (HFD) consumption programs β-cell dysfunction in female rat offspring, together with transcriptome alterations in islets. Here we investigated the retroperitoneal white adipose tissue (RpWAT) transcriptome using gene and pathway enrichment and pathway analysis to determine whether commonly affected network topologies exist between these two metabolically related tissues. In RpWAT, 5108 genes were differentially expressed due to a paternal HFD; the top 5 significantly enriched networks identified by pathway analysis in offspring of HFD fathers compared with those of fathers fed control diet were: mitochondrial and cellular response to stress, telomerase signaling, cell death and survival, cell cycle, cellular growth and proliferation, and cancer. A total of 187 adipose olfactory receptor genes were down-regulated. Interrogation against the islet transcriptome identified specific gene networks and pathways, including olfactory receptor genes that were similarly affected in both tissues (411 common genes, P<0.05). In particular, we highlight a common molecular network, cell cycle and cancer, with the same hub gene, Myc, suggesting early onset developmental changes that persist, shared responses to programmed systemic factors, or crosstalk between tissues. Thus, paternal HFD consumption triggers unique gene signatures, consistent with premature aging and chronic degenerative disorders, in both RpWAT and pancreatic islets of daughters.

    Original languageEnglish
    Pages (from-to)1830-1841
    Number of pages12
    JournalFASEB Journal
    Volume28
    Issue number4
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    High Fat Diet
    Nutrition
    Islets of Langerhans
    Transcriptome
    Rats
    Genes
    Fats
    Tissue
    White Adipose Tissue
    Intra-Abdominal Fat
    Odorant Receptors
    Fathers
    Cell Cycle
    Premature Aging
    myc Genes
    Gene Regulatory Networks
    Telomerase
    Cells
    Nuclear Family
    Neoplasms

    Keywords

    • Chronic degenerative disorder
    • Developmental programming
    • Obesity
    • Olfactory receptor genes
    • Premature aging

    ASJC Scopus subject areas

    • Biochemistry
    • Biotechnology
    • Genetics
    • Molecular Biology

    Cite this

    Paternal high-fat diet consumption induces common changes in the transcriptomes of retroperitoneal adipose and pancreatic islet tissues in female rat offspring. / Ng, Sheau Fang; Lin, Ruby C Y; Maloney, Christopher A.; Youngson, Neil A.; Owens, Julie A.; Morris, Margaret J.

    In: FASEB Journal, Vol. 28, No. 4, 2014, p. 1830-1841.

    Research output: Contribution to journalArticle

    Ng, Sheau Fang ; Lin, Ruby C Y ; Maloney, Christopher A. ; Youngson, Neil A. ; Owens, Julie A. ; Morris, Margaret J. / Paternal high-fat diet consumption induces common changes in the transcriptomes of retroperitoneal adipose and pancreatic islet tissues in female rat offspring. In: FASEB Journal. 2014 ; Vol. 28, No. 4. pp. 1830-1841.
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