Partial purification and isoelectric focusing patterns of the buffalo (Bubalus bubalis) and the kedah-kelantan cattle (Bos indicus) glutathione transferases

N. A. Shamaan, I. Yunus, H. Mahbut, W. Z. Wan Ngah

    Research output: Contribution to journalArticle

    Abstract

    1. 1. Glutathione transferases from the liver, lung and kidney tissues of the buffalo (Bubalus bubalis) and the Kedah-Kelantan cattle (Bos indicus were partially purified by ammonium sulphate precipitation and Sephadex G-75 gel filtration. 2. 2. Liver tissue contains the highest enzyme activity when compared to the lung and kidney tissues. 3. 3. The activity in cattle is higher than that in the buffalo. 4. 4. Isoelectric focusing separates the activities into the acidic, near neutral and basic fractions. 5. 5. The focused patterns are different for each of the tissues and in each of the species investigated.

    Original languageEnglish
    Pages (from-to)259-263
    Number of pages5
    JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
    Volume100
    Issue number2
    DOIs
    Publication statusPublished - 1991

    Fingerprint

    Buffaloes
    Isoelectric Focusing
    Glutathione Transferase
    Purification
    Tissue
    Liver
    Kidney
    Lung
    Ammonium Sulfate
    Enzyme activity
    Gel Chromatography
    Gels
    Enzymes

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology

    Cite this

    Partial purification and isoelectric focusing patterns of the buffalo (Bubalus bubalis) and the kedah-kelantan cattle (Bos indicus) glutathione transferases. / Shamaan, N. A.; Yunus, I.; Mahbut, H.; Wan Ngah, W. Z.

    In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 100, No. 2, 1991, p. 259-263.

    Research output: Contribution to journalArticle

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