Parameter optimization in the extraction of sea cucumber (Holothuria Scabra J) as a source of testosterone

Kurnia Harlina Dewi, Masturah Markom, Wan Ramli Wan Daud

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Testosterone, the steroid hormone used in sex reversal and as an aphrodisiac, is produced not only by sea cucumber, but many other organisms too. This research was aimed at optimizing conventional extraction of testosterone from sea cucumber, looking at method, type of solvent, solvent ratio, and temperature for isolation. The results showed that extraction by reflux produced the highest testosterone content, followed by soxhlet extraction. The lowest yield was produced by maceration extraction. The solvents selected were acetone, methanol, methanol/chloroform mixture (1:2) and chloroform. The highest result was obtained by methanol/chloroform (0.2728 mg /100g dry weight (dw)), followed by acetone (0.2623 mg), chloroform (0.1606 mg) and methanol (0.0920 mg). Ratios of material:solvent used were 1:1, 1:2 and 1:3 respectively. Results improved as the amount of solvent was increased, so a ratio of 1:3 was most successful and 1:1 least successful. The effects of temperature and time on scale-up reflux extraction were also studied. Scale-up reflux extraction of 3000 ml showed that raising the temperature increases the percentage of sea cucumber extract.

Original languageEnglish
Title of host publicationAdvanced Materials Research
Pages1358-1365
Number of pages8
Volume233-235
DOIs
Publication statusPublished - 2011
Event2011 International Conference on Chemical Engineering and Advanced Materials, CEAM 2011 - Changsha
Duration: 28 May 201130 May 2011

Publication series

NameAdvanced Materials Research
Volume233-235
ISSN (Print)10226680

Other

Other2011 International Conference on Chemical Engineering and Advanced Materials, CEAM 2011
CityChangsha
Period28/5/1130/5/11

Fingerprint

Chlorine compounds
Methanol
Acetone
Steroid hormones
Temperature

Keywords

  • Maceration
  • Reflux
  • Sea cucumber
  • Soxhlet
  • Testosterone

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Parameter optimization in the extraction of sea cucumber (Holothuria Scabra J) as a source of testosterone. / Dewi, Kurnia Harlina; Markom, Masturah; Wan Daud, Wan Ramli.

Advanced Materials Research. Vol. 233-235 2011. p. 1358-1365 (Advanced Materials Research; Vol. 233-235).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Dewi, KH, Markom, M & Wan Daud, WR 2011, Parameter optimization in the extraction of sea cucumber (Holothuria Scabra J) as a source of testosterone. in Advanced Materials Research. vol. 233-235, Advanced Materials Research, vol. 233-235, pp. 1358-1365, 2011 International Conference on Chemical Engineering and Advanced Materials, CEAM 2011, Changsha, 28/5/11. https://doi.org/10.4028/www.scientific.net/AMR.233-235.1358
Dewi, Kurnia Harlina ; Markom, Masturah ; Wan Daud, Wan Ramli. / Parameter optimization in the extraction of sea cucumber (Holothuria Scabra J) as a source of testosterone. Advanced Materials Research. Vol. 233-235 2011. pp. 1358-1365 (Advanced Materials Research).
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