Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet

Ku Zaifah Norsidah, Ahmad Yusof Asmadi, Ayob Azizi, Faizah Othman, Kamisah Yusof

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Oxidative stress contributes to cardiovascular diseases. We aimed to study the effects of palm tocotrienol-rich fraction (TRF) on plasma homocysteine and cardiac oxidative stress in rats fed with a highmethionine diet. Forty-two male Wistar rats were divided into six groups. The first group was the control. Groups 2-6 were fed 1 % methionine diet for 10 weeks. From week 6 onward, folate (8 mg/kg diet) or palm TRF (30, 60 and 150 mg/kg diet) was added into the diet of groups 3, 4, 5 and 6. The rats were then killed. Palm TRF at 150 mg/kg and folate supplementation prevented the increase in plasma total homocysteine (4.14±0.33 and 4.30±0.26 vs 5.49±0.25 mmol/L, p<0.05) induced by a high-methionine diet. The increased heart thiobarbituric acid reactive substance in rats fed with high-methionine diet was also prevented by the supplementations of palm TRF (60 and 150 mg/kg) and folate. The highmethionine group had a lower glutathione peroxidase activity (49±3 vs 69±4 pmol/mg protein/min) than the control group. This reduction was reversed by palm TRF at 60 and 150 mg/kg diet (p<0.05), but not by folate. Catalase and superoxide dismutase activities were unaffected by both methionine and vitamin supplementations. In conclusion, palm TRF was comparable to folate in reducing high-methionine diet-induced hyperhomocysteinemia and oxidative stress in the rats' hearts. However, palm TRF was more effective than folate in preserving the heart glutathione peroxidase enzyme activity.

Original languageEnglish
Pages (from-to)441-449
Number of pages9
JournalJournal of Physiology and Biochemistry
Volume69
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

Tocotrienols
Oxidative stress
Homocysteine
Nutrition
Methionine
Rats
Oxidative Stress
Diet
Plasmas
Folic Acid
Glutathione Peroxidase
Hyperhomocysteinemia
Control Groups
Thiobarbituric Acid Reactive Substances
Enzyme activity
Vitamins
Catalase
Superoxide Dismutase
Wistar Rats
Cardiovascular Diseases

Keywords

  • Antioxidant enzymes
  • Folate
  • Homocysteine
  • Methionine
  • Oxidative stress
  • Tocotrienol

ASJC Scopus subject areas

  • Physiology
  • Biochemistry

Cite this

Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet. / Norsidah, Ku Zaifah; Asmadi, Ahmad Yusof; Azizi, Ayob; Othman, Faizah; Yusof, Kamisah.

In: Journal of Physiology and Biochemistry, Vol. 69, No. 3, 2013, p. 441-449.

Research output: Contribution to journalArticle

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AU - Yusof, Kamisah

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