Palm oil-derived phytosterol: Glutathione antioxidant status in rats exposed to carbon tetrachloride

Research output: Contribution to journalArticle

Abstract

The aim of this study was to assess the antioxidant effect of phytosterol from palm oil by studying its ability to improve antioxidant status of rats induced with oxidative stress by carbon tetrachloride (CCl4). The rats were divided into four groups of normal control (NC), carbon tetrachloride (CCl4), phytosterol (P) and phytosterol plus carbon tetrachloride (P+CCl4). The P and P+CCl4 groups received weekly phytosterol pre-treatment via subcutaneous injections at 140 mg/kg rat weight for 5 weeks while the NC and CCl4 groups only received olive oil (vehicle). Carbon tetrachloride at the dose determined by a preliminary study was given as single oral dose to induce lipid peroxidation in the CCl4 and P+CCl4 groups. After 24 hours, the rats were sacrificed and the heart, liver, kidney and lung were isolated for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) levels. Carbon tetrachloride caused significant reduction in the GSH:GSSH ratio in all major organs. Phytosterol pre-treatment as in the P+CCl4 group significantly increased the GSH:GSSG ratio in major organs. The present findings indicate that phytosterols keep tissue glutathione concentration in normal levels which may indicate improving antioxidant status in major organs of the rats treated with carbon tetrachloride.

Original languageEnglish
Pages (from-to)90-95
Number of pages6
JournalJournal of Applied Pharmaceutical Science
Volume6
Issue number8
DOIs
Publication statusPublished - 2016

Fingerprint

Phytosterols
Carbon Tetrachloride
Glutathione
Antioxidants
Glutathione Disulfide
Control Groups
Subcutaneous Injections
Lipid Peroxidation
palm oil
Oxidative Stress
Kidney
Weights and Measures
Lung
Liver
Therapeutics

Keywords

  • Antioxidant status
  • Carbon tetrachloride
  • Glutathione
  • phytosterol

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Medicine (miscellaneous)
  • Pharmacology (medical)

Cite this

@article{6535de07b81b4ec5bc95f8eb6f723762,
title = "Palm oil-derived phytosterol: Glutathione antioxidant status in rats exposed to carbon tetrachloride",
abstract = "The aim of this study was to assess the antioxidant effect of phytosterol from palm oil by studying its ability to improve antioxidant status of rats induced with oxidative stress by carbon tetrachloride (CCl4). The rats were divided into four groups of normal control (NC), carbon tetrachloride (CCl4), phytosterol (P) and phytosterol plus carbon tetrachloride (P+CCl4). The P and P+CCl4 groups received weekly phytosterol pre-treatment via subcutaneous injections at 140 mg/kg rat weight for 5 weeks while the NC and CCl4 groups only received olive oil (vehicle). Carbon tetrachloride at the dose determined by a preliminary study was given as single oral dose to induce lipid peroxidation in the CCl4 and P+CCl4 groups. After 24 hours, the rats were sacrificed and the heart, liver, kidney and lung were isolated for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) levels. Carbon tetrachloride caused significant reduction in the GSH:GSSH ratio in all major organs. Phytosterol pre-treatment as in the P+CCl4 group significantly increased the GSH:GSSG ratio in major organs. The present findings indicate that phytosterols keep tissue glutathione concentration in normal levels which may indicate improving antioxidant status in major organs of the rats treated with carbon tetrachloride.",
keywords = "Antioxidant status, Carbon tetrachloride, Glutathione, phytosterol",
author = "{Mohd Fahami}, {Nur Azlina} and {Mohd Saad}, Qodriyah and Norazlina Mohamed and Kamisah Yusof and Shuid, {Ahmad Nazrun} and Marzuki Alini",
year = "2016",
doi = "10.7324/JAPS.2016.60814",
language = "English",
volume = "6",
pages = "90--95",
journal = "Journal of Applied Pharmaceutical Science",
issn = "2231-3354",
publisher = "MediPoeia",
number = "8",

}

TY - JOUR

T1 - Palm oil-derived phytosterol

T2 - Glutathione antioxidant status in rats exposed to carbon tetrachloride

AU - Mohd Fahami, Nur Azlina

AU - Mohd Saad, Qodriyah

AU - Mohamed, Norazlina

AU - Yusof, Kamisah

AU - Shuid, Ahmad Nazrun

AU - Alini, Marzuki

PY - 2016

Y1 - 2016

N2 - The aim of this study was to assess the antioxidant effect of phytosterol from palm oil by studying its ability to improve antioxidant status of rats induced with oxidative stress by carbon tetrachloride (CCl4). The rats were divided into four groups of normal control (NC), carbon tetrachloride (CCl4), phytosterol (P) and phytosterol plus carbon tetrachloride (P+CCl4). The P and P+CCl4 groups received weekly phytosterol pre-treatment via subcutaneous injections at 140 mg/kg rat weight for 5 weeks while the NC and CCl4 groups only received olive oil (vehicle). Carbon tetrachloride at the dose determined by a preliminary study was given as single oral dose to induce lipid peroxidation in the CCl4 and P+CCl4 groups. After 24 hours, the rats were sacrificed and the heart, liver, kidney and lung were isolated for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) levels. Carbon tetrachloride caused significant reduction in the GSH:GSSH ratio in all major organs. Phytosterol pre-treatment as in the P+CCl4 group significantly increased the GSH:GSSG ratio in major organs. The present findings indicate that phytosterols keep tissue glutathione concentration in normal levels which may indicate improving antioxidant status in major organs of the rats treated with carbon tetrachloride.

AB - The aim of this study was to assess the antioxidant effect of phytosterol from palm oil by studying its ability to improve antioxidant status of rats induced with oxidative stress by carbon tetrachloride (CCl4). The rats were divided into four groups of normal control (NC), carbon tetrachloride (CCl4), phytosterol (P) and phytosterol plus carbon tetrachloride (P+CCl4). The P and P+CCl4 groups received weekly phytosterol pre-treatment via subcutaneous injections at 140 mg/kg rat weight for 5 weeks while the NC and CCl4 groups only received olive oil (vehicle). Carbon tetrachloride at the dose determined by a preliminary study was given as single oral dose to induce lipid peroxidation in the CCl4 and P+CCl4 groups. After 24 hours, the rats were sacrificed and the heart, liver, kidney and lung were isolated for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) levels. Carbon tetrachloride caused significant reduction in the GSH:GSSH ratio in all major organs. Phytosterol pre-treatment as in the P+CCl4 group significantly increased the GSH:GSSG ratio in major organs. The present findings indicate that phytosterols keep tissue glutathione concentration in normal levels which may indicate improving antioxidant status in major organs of the rats treated with carbon tetrachloride.

KW - Antioxidant status

KW - Carbon tetrachloride

KW - Glutathione

KW - phytosterol

UR - http://www.scopus.com/inward/record.url?scp=84985961274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84985961274&partnerID=8YFLogxK

U2 - 10.7324/JAPS.2016.60814

DO - 10.7324/JAPS.2016.60814

M3 - Article

AN - SCOPUS:84985961274

VL - 6

SP - 90

EP - 95

JO - Journal of Applied Pharmaceutical Science

JF - Journal of Applied Pharmaceutical Science

SN - 2231-3354

IS - 8

ER -