Paenibacillus alvei (AN5) and gramicidin a antimicrobial peptide activities

Sahlil Miraz Mohamed Rafie, Darman Nordin, Nurina Anuar

Research output: Contribution to journalArticle

Abstract

Antibacterial resistance is a major problem faced in public health. Antimicrobial peptides (AMPs) has gained attention as potential for antiinfectives due to their wide broad activity. AMPs are part of universal defense system in almost all living organism. In this study, antimicrobial activity of AMP crude extract from Paenibacillus alvei AN5 (crude AN5) and commercial AMP (Gramicidin A, gA) were tested against Gram positive bacteria, Staphylococcus aureus and Gram negative bacteria, Escherichia coli using broth dilution method. The results express in turbidity measurement showed reduction of S. aureus growth by 64% with treatment of gA and 50% with treatment of crude AN5. For E. coli, the percentages of reduction are 49% and 43%. For the effect of different concentrations of AMP, S. aureus was inhibited at 10% v/v of gA, 100% for crude AN5 and E. coli 50% gA, 100% crude AN5. Crude AN5 and gA were capabale of inhibiting both Gram negative and positive bacteria.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalMalaysian Applied Biology
Volume47
Issue number5
Publication statusPublished - 1 Nov 2018

Fingerprint

Paenibacillus alvei
gramicidin
Paenibacillus
Gramicidin
antimicrobial peptides
Peptides
Staphylococcus aureus
Gram-positive bacteria
Escherichia coli
Gram-negative bacteria
Gram-Positive Bacteria
Gram-Negative Bacteria
antibiotic resistance
turbidity
Complex Mixtures
public health
anti-infective agents
gastrofenzin
Public Health
organisms

Keywords

  • Antimicrobial activity
  • Gramicidin A
  • P. Alvei AN5

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Paenibacillus alvei (AN5) and gramicidin a antimicrobial peptide activities. / Rafie, Sahlil Miraz Mohamed; Nordin, Darman; Anuar, Nurina.

In: Malaysian Applied Biology, Vol. 47, No. 5, 01.11.2018, p. 261-267.

Research output: Contribution to journalArticle

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