Oxidative stress in fetal lambs exposed to intra-amniotic endotoxin in a chorioamnionitis model

Cheah Fook Choe, Alan H. Jobe, Timothy J. Moss, John P. Newnham, Suhas G. Kallapur

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Chorioamnionitis is a risk factor for the development of bronchopulmonary dysplasia. Endotoxin-induced oxidative stress to the fetus in the uniquely hypoxic intrauterine environment has not been reported. Using a model of chorioamnionitis, we measured markers of pulmonary and systemic oxidant exposures in fetal lambs at 124 d gestation (term = 150 d) exposed to 10 mg intra-amniotic endotoxin 2 d (n = 6) or 7 d (n = 6) before delivery, or saline as controls (n = 9). The 7 d endotoxin-exposed animals had 3-fold higher protein carbonyls (0.66 ± 0.46 versus 0.23 ± 0.14 nmol/mg protein) and 10-fold greater myeloperoxidase activity (2.38 ± 1.87 versus 0.27 ± 0.18 nM) in the bronchoalveolar lavage fluid (BALF), suggestive of neutrophil-derived oxidant activity. However, in the lung tissue, protein carbonyls, superoxide dismutase, and peroxiredoxin 1 were not different between groups. The expression of peroxiredoxin 1 was prominent, primarily in the peri-bronchiolar epithelium. Notably, evidence of oxidant exposure was minimal at 2 d when BALF inflammatory cells, lung IL-1β, and IL-8 were highest. Intra-amniotic endotoxin induced systemic oxidative stress as plasma protein carbonyl was elevated at 7 d (0.14 ± 0.04 nmol/mg protein; p = 0.005). Surfactant protein A and B mRNAs were highest at 2 d, suggesting that oxidative stress did not contribute to the lung maturation response. A modest lung oxidative stress in chorioamnionitis could contribute to bronchopulmonary dysplasia.

Original languageEnglish
Pages (from-to)274-279
Number of pages6
JournalPediatric Research
Volume63
Issue number3
DOIs
Publication statusPublished - Mar 2008
Externally publishedYes

Fingerprint

Chorioamnionitis
Endotoxins
Oxidative Stress
Lung
Oxidants
Peroxiredoxins
Bronchopulmonary Dysplasia
Bronchoalveolar Lavage Fluid
Proteins
Pulmonary Surfactant-Associated Protein A
Interleukin-8
Interleukin-1
Peroxidase
Blood Proteins
Neutrophils
Fetus
Epithelium
Pregnancy
Messenger RNA

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Oxidative stress in fetal lambs exposed to intra-amniotic endotoxin in a chorioamnionitis model. / Fook Choe, Cheah; Jobe, Alan H.; Moss, Timothy J.; Newnham, John P.; Kallapur, Suhas G.

In: Pediatric Research, Vol. 63, No. 3, 03.2008, p. 274-279.

Research output: Contribution to journalArticle

Fook Choe, Cheah ; Jobe, Alan H. ; Moss, Timothy J. ; Newnham, John P. ; Kallapur, Suhas G. / Oxidative stress in fetal lambs exposed to intra-amniotic endotoxin in a chorioamnionitis model. In: Pediatric Research. 2008 ; Vol. 63, No. 3. pp. 274-279.
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