Oxidative stress and DNA damage in streptozotocin-induced diabetic rats

Effects of alpha lipoic acid

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3 Citations (Scopus)

Abstract

The aim of present study was to investigate the effect of alpha lipoic acid (ALA) supplementation on oxidative stress and DNA damage in streptozotocin-induced diabetic rats. The diabetic rats were divided into two groups; supplemented with ALA (100 mg/kg/day) and non-supplemented with ALA (No Suppl group). Non-diabetic rats (NDM) formed the control group. Following eight weeks of supplementation, fasting blood glucose (FBG) and HBA1c in ALA-supplemented rats was found to be significantly lower than the No Suppl diabetic rats. ALA supplementation also significantly increased plasma superoxide dismutase (SOD) activity and vitamin C level compared to the No Suppl group. The increased in plasma malondealdehyde (MDA) and 4-hydroxynonenal (4-HNE) levels also were inhibited. At the same time, the percentages of comet tail and tail moment of peripheral lymphocytes which indicate the oxidative DNA damage were significantly reduced in the ALA-supplementation group. These results suggest that ALA has protective effects against oxidative stress by increasing antioxidant status and reducing lipid peroxidation and DNA damage in streptozotocin-induced diabetic rats.

Original languageEnglish
Pages (from-to)622-627
Number of pages6
JournalJournal of Biological Sciences
Volume8
Issue number3
DOIs
Publication statusPublished - 2008

Fingerprint

Thioctic Acid
Streptozocin
DNA Damage
Oxidative Stress
Tail
Lipid Peroxidation
Ascorbic Acid
Superoxide Dismutase
Blood Glucose
Fasting
Antioxidants
Lymphocytes
Control Groups

Keywords

  • Alpha lipoic acid
  • Antioxidants
  • Diabetes mellitus
  • DNA damage
  • Free radicals
  • Oxidative stress

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

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title = "Oxidative stress and DNA damage in streptozotocin-induced diabetic rats: Effects of alpha lipoic acid",
abstract = "The aim of present study was to investigate the effect of alpha lipoic acid (ALA) supplementation on oxidative stress and DNA damage in streptozotocin-induced diabetic rats. The diabetic rats were divided into two groups; supplemented with ALA (100 mg/kg/day) and non-supplemented with ALA (No Suppl group). Non-diabetic rats (NDM) formed the control group. Following eight weeks of supplementation, fasting blood glucose (FBG) and HBA1c in ALA-supplemented rats was found to be significantly lower than the No Suppl diabetic rats. ALA supplementation also significantly increased plasma superoxide dismutase (SOD) activity and vitamin C level compared to the No Suppl group. The increased in plasma malondealdehyde (MDA) and 4-hydroxynonenal (4-HNE) levels also were inhibited. At the same time, the percentages of comet tail and tail moment of peripheral lymphocytes which indicate the oxidative DNA damage were significantly reduced in the ALA-supplementation group. These results suggest that ALA has protective effects against oxidative stress by increasing antioxidant status and reducing lipid peroxidation and DNA damage in streptozotocin-induced diabetic rats.",
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T1 - Oxidative stress and DNA damage in streptozotocin-induced diabetic rats

T2 - Effects of alpha lipoic acid

AU - Budin, Siti Balkis

AU - Rajab, Nor Fadilah

AU - Ezlan, A.

AU - Osman, Khairul

AU - Mokhtar, A. B.

AU - Mohamed, Jamaludin

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N2 - The aim of present study was to investigate the effect of alpha lipoic acid (ALA) supplementation on oxidative stress and DNA damage in streptozotocin-induced diabetic rats. The diabetic rats were divided into two groups; supplemented with ALA (100 mg/kg/day) and non-supplemented with ALA (No Suppl group). Non-diabetic rats (NDM) formed the control group. Following eight weeks of supplementation, fasting blood glucose (FBG) and HBA1c in ALA-supplemented rats was found to be significantly lower than the No Suppl diabetic rats. ALA supplementation also significantly increased plasma superoxide dismutase (SOD) activity and vitamin C level compared to the No Suppl group. The increased in plasma malondealdehyde (MDA) and 4-hydroxynonenal (4-HNE) levels also were inhibited. At the same time, the percentages of comet tail and tail moment of peripheral lymphocytes which indicate the oxidative DNA damage were significantly reduced in the ALA-supplementation group. These results suggest that ALA has protective effects against oxidative stress by increasing antioxidant status and reducing lipid peroxidation and DNA damage in streptozotocin-induced diabetic rats.

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