Oxidative damage and antioxidant status in patients with cervical intraepithelial neoplasia and carcinoma of the cervix

Mee Lee Looi, Zailani Hatta Ahmad Zailani Hatta, Ahmad Zailani Hatta Mohd Dali, Siti Aishah Md Ali, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Free radicals that induced lipid peroxidation and DNA damage have been implicated in many diseases including cancer. Cellular antioxidant defense plays an important role in neoplastic disease to counteract oxidative damage. This study aims to investigate the status of oxidative damage by measuring plasma malondialdehyde (MDA) level and urinary 8-hydroxydeoxyguanosine (8-OHdG), and the level of antioxidant enzymes superoxide dismutase, glutathione peroxidase, and catalase in patients with cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC) of the cervix. Urinary 8-OHdG was measured by an enzyme-linked immunosorbent assay kit. MDA and antioxidant enzyme activities were determined by high-performance liquid chromatography and spectrophotometry, respectively. Eighty patients with CIN and SCC of the cervix were recruited and compared with normal controls. Urinary 8-OHdG/creatinine ratio did not show any significant changes in any disease status studied as compared with controls (P= 0.803). Plasma MDA was found to be increased in CIN and SCC patients when compared with controls (P= 0.002). Glutathione peroxidase activity was increased (P=0.0001) whereas superoxide dismutase and catalase activity was decreased (P=0.019 and 0.0001, respectively) in both CIN and SCC patients when compared with controls. Urinary 8-OHdG may not be a good marker for enhanced oxidative stress in cervical cancer. Oxidative damage as demonstrated by the level of MDA is markedly increased in CIN and SCC patients with changes of enzymatic antioxidants observed.

Original languageEnglish
Pages (from-to)555-560
Number of pages6
JournalEuropean Journal of Cancer Prevention
Volume17
Issue number6
DOIs
Publication statusPublished - Nov 2008

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Cervical Intraepithelial Neoplasia
Carcinoma in Situ
Cervix Uteri
Squamous Cell Carcinoma
Malondialdehyde
Antioxidants
Glutathione Peroxidase
Catalase
Superoxide Dismutase
Spectrophotometry
Enzymes
Uterine Cervical Neoplasms
Lipid Peroxidation
DNA Damage
Free Radicals
Creatinine
Oxidative Stress
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Neoplasms

Keywords

  • 8-Deoxyhydroxyguanosine
  • Antioxidant enzymes
  • Cervical carcinoma
  • Cervical intraepithelial neoplasia
  • Malondialdehyde

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Epidemiology
  • Public Health, Environmental and Occupational Health

Cite this

Looi, M. L., Ahmad Zailani Hatta, Z. H., Dali, A. Z. H. M., Ali, S. A. M., Ngah, W. Z. W., & Mohd Yusof, Y. A. (2008). Oxidative damage and antioxidant status in patients with cervical intraepithelial neoplasia and carcinoma of the cervix. European Journal of Cancer Prevention, 17(6), 555-560. https://doi.org/10.1097/CEJ.0b013e328305a10b

Oxidative damage and antioxidant status in patients with cervical intraepithelial neoplasia and carcinoma of the cervix. / Looi, Mee Lee; Ahmad Zailani Hatta, Zailani Hatta; Dali, Ahmad Zailani Hatta Mohd; Ali, Siti Aishah Md; Ngah, Wan Zurinah Wan; Mohd Yusof, Yasmin Anum.

In: European Journal of Cancer Prevention, Vol. 17, No. 6, 11.2008, p. 555-560.

Research output: Contribution to journalArticle

Looi, Mee Lee ; Ahmad Zailani Hatta, Zailani Hatta ; Dali, Ahmad Zailani Hatta Mohd ; Ali, Siti Aishah Md ; Ngah, Wan Zurinah Wan ; Mohd Yusof, Yasmin Anum. / Oxidative damage and antioxidant status in patients with cervical intraepithelial neoplasia and carcinoma of the cervix. In: European Journal of Cancer Prevention. 2008 ; Vol. 17, No. 6. pp. 555-560.
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