Overexpression of Auxin Binding Protein 57 from Rice (Oryza sativa L.) Increased Drought and Salt Tolerance in Transgenic Arabidopsis thaliana

L. W. Tan, C. S. Tan, A. R. Zuraida, H. M. Hossein, Hoe Han Goh, Ismanizan Ismail, Zamri Zainal

Research output: Contribution to journalConference article

Abstract

Auxin plays important roles in many aspects of plant growth and development. It is known that auxin binds to auxin-binding protein (Abp57) and activates the plasma membrane H+-ATPases. In order to elucidate the biological function of rice Abp57 in plants, overexpression transgenic arabidopsis lines were generated. We found overexpression transgenic arabidopsis harbouring rice Abp57 showed tolerance to osmotic stress. Phenotypic transgenic arabidopsis showed longer roots than the wildtype when grown on 1/2 MS media supplemented with various concentrations of NaCl or PEG-6000. Besides, transgenic arabidopsis has relatively lower water loss rate, higher chlorophyll content and higher biomass when growing under osmotic stress compared to the wildtype. These results suggested that Abp57 has important roles in giving tolerance to arabidopsis against drought and salt stresses.

Original languageEnglish
Article number012038
JournalIOP Conference Series: Earth and Environmental Science
Volume197
Issue number1
DOIs
Publication statusPublished - 2 Nov 2018
Event2nd International Conference on Biosciences, ICoBio 2017 - Bogor, Indonesia
Duration: 8 Aug 201710 Aug 2017

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rice
tolerance
drought
salt
protein
transgenic plant
growth and development
chlorophyll
membrane
plasma
biomass
water
loss
rate

Keywords

  • Abiotic stress tolerance
  • auxin
  • NaCl
  • osmotic stress

ASJC Scopus subject areas

  • Environmental Science(all)
  • Earth and Planetary Sciences(all)

Cite this

Overexpression of Auxin Binding Protein 57 from Rice (Oryza sativa L.) Increased Drought and Salt Tolerance in Transgenic Arabidopsis thaliana. / Tan, L. W.; Tan, C. S.; Zuraida, A. R.; Hossein, H. M.; Goh, Hoe Han; Ismail, Ismanizan; Zainal, Zamri.

In: IOP Conference Series: Earth and Environmental Science, Vol. 197, No. 1, 012038, 02.11.2018.

Research output: Contribution to journalConference article

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AU - Tan, L. W.

AU - Tan, C. S.

AU - Zuraida, A. R.

AU - Hossein, H. M.

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AU - Ismail, Ismanizan

AU - Zainal, Zamri

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