Overexpression of a Rice Monosaccharide Transporter Gene (OsMST6) Confers Enhanced Tolerance to Drought and Salinity Stress in Arabidopsis thaliana

Hossein Hosseini Monfared, Jin Kiat Chew, Parisa Azizi, Gang Ping Xue, Su Fang Ee, Saeid Kadkhodaei, Pouya Hedayati, Ismanizan Ismail, Zamri Zainal

Research output: Contribution to journalArticle

Abstract

Monosaccharide transporter (MSTs) is a large family of integral membrane proteins that plays a crucial role in cell-to-cell and long-distance distribution of sugars throughout the plant. It has long been regarded as one of the most essential components in a myriad of plant physiological functions such as carbohydrate partitioning, sugar signaling, and environmental stress response. In the present study, an in vivo functional analysis of a rice monosaccharide transporter gene, namely OsMST6, has been conducted using a transgenic approach to understand its role in plant responses to abiotic stress. Physiological traits analysis indicated that the transgenic lines had a relatively lower water loss rate, higher relative water content (1.3–2.2-fold), increased cell membrane stability (1.5–2-fold), and higher total soluble sugar content (2.8–3.5-fold) compared to the parental lines. Furthermore, the overexpression of OsMST6 was also found to enhance both drought and salt tolerance in transgenic Arabidopsis thaliana. Taken together, these results suggested that OsMST6 may be involved in the adaptation of rice plant to water stress by maintaining the water status of plant cells. This study provides insight into the function of OsMST6, which may have potential application in the generation of transgenic abiotic stress-tolerant plants.

Original languageEnglish
JournalPlant Molecular Biology Reporter
DOIs
Publication statusAccepted/In press - 1 Jan 2020

Fingerprint

Monosaccharides
Droughts
Salinity
monosaccharides
Arabidopsis
salt stress
transporters
water stress
Arabidopsis thaliana
rice
genetically modified organisms
Genes
genes
abiotic stress
Water
Salt-Tolerance
sugars
Plant Cells
cells
in vivo studies

Keywords

  • Drought tolerance
  • Monosaccharide transporter
  • Oryza sativa
  • Transgenic Arabidopsis

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

Cite this

Overexpression of a Rice Monosaccharide Transporter Gene (OsMST6) Confers Enhanced Tolerance to Drought and Salinity Stress in Arabidopsis thaliana. / Monfared, Hossein Hosseini; Chew, Jin Kiat; Azizi, Parisa; Xue, Gang Ping; Ee, Su Fang; Kadkhodaei, Saeid; Hedayati, Pouya; Ismail, Ismanizan; Zainal, Zamri.

In: Plant Molecular Biology Reporter, 01.01.2020.

Research output: Contribution to journalArticle

Monfared, Hossein Hosseini ; Chew, Jin Kiat ; Azizi, Parisa ; Xue, Gang Ping ; Ee, Su Fang ; Kadkhodaei, Saeid ; Hedayati, Pouya ; Ismail, Ismanizan ; Zainal, Zamri. / Overexpression of a Rice Monosaccharide Transporter Gene (OsMST6) Confers Enhanced Tolerance to Drought and Salinity Stress in Arabidopsis thaliana. In: Plant Molecular Biology Reporter. 2020.
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