Osteogenic potential of in situ TiO2 nanowire surfaces formed by thermal oxidation of titanium alloy substrate

A. W. Tan, R. Ismail, Chua Kien Hui, R. Ahmad, S. A. Akbar, B. Pingguan-Murphy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Titanium dioxide (TiO2) nanowire surface structures were fabricated in situ by a thermal oxidation process, and their ability to enhance the osteogenic potential of primary osteoblasts was investigated. Human osteoblasts were isolated from nasal bone and cultured on a TiO2 nanowires coated substrate to assess its in vitro cellular interaction. Bare featureless Ti-6Al-4V substrate was used as a control surface. Initial cell adhesion, cell proliferation, cell differentiation, cell mineralization, and osteogenic related gene expression were examined on the TiO2 nanowire surfaces as compared to the control surfaces after 2 weeks of culturing. Cell adhesion and cell proliferation were assayed by field emission scanning electron microscope (FESEM) and Alamar Blue reduction assay, respectively. The nanowire surfaces promoted better cell adhesion and spreading than the control surface, as well as leading to higher cell proliferation. Our results showed that osteoblasts grown onto the TiO2 nanowire surfaces displayed significantly higher production levels of alkaline phosphatase (ALP), extracellular (ECM) mineralization and genes expression of runt-related transcription factor (Runx2), bone sialoprotein (BSP), ostoepontin (OPN) and osteocalcin (OCN) compared to the control surfaces. This suggests the potential use of such surface modification on Ti-6Al-4V substrates as a promising means to improve the osteointegration of titanium based implants.

Original languageEnglish
Pages (from-to)161-170
Number of pages10
JournalApplied Surface Science
Volume320
DOIs
Publication statusPublished - 30 Nov 2014

Fingerprint

Titanium alloys
Control surfaces
Nanowires
Osteoblasts
Cell adhesion
Cell proliferation
Oxidation
Substrates
Gene expression
Bone
Integrin-Binding Sialoprotein
Military electronic countermeasures
Transcription factors
Osteocalcin
Phosphatases
Titanium
Surface structure
Field emission
Titanium dioxide
Alkaline Phosphatase

Keywords

  • Nanowires
  • Osteogenic
  • Surface modification
  • Thermal oxidation
  • Titanium dioxide

ASJC Scopus subject areas

  • Surfaces, Coatings and Films

Cite this

Osteogenic potential of in situ TiO2 nanowire surfaces formed by thermal oxidation of titanium alloy substrate. / Tan, A. W.; Ismail, R.; Kien Hui, Chua; Ahmad, R.; Akbar, S. A.; Pingguan-Murphy, B.

In: Applied Surface Science, Vol. 320, 30.11.2014, p. 161-170.

Research output: Contribution to journalArticle

Tan, A. W. ; Ismail, R. ; Kien Hui, Chua ; Ahmad, R. ; Akbar, S. A. ; Pingguan-Murphy, B. / Osteogenic potential of in situ TiO2 nanowire surfaces formed by thermal oxidation of titanium alloy substrate. In: Applied Surface Science. 2014 ; Vol. 320. pp. 161-170.
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