Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation

Cin Kong, Man Wah Tan, Sheila Nathan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Amidst growing concerns over the spread of antibiotic-resistant Staphylococcus aureus strains, the identification of alternative therapeutic molecules has become paramount. Previously, we utilized a Caenorhabditis elegans-S. aureus screening platform to identify potential anti-infective agents from a collection of natural extracts and synthetic compounds. One of the hits obtained from the screen was the aqueous extract of Orthosiphon stamineus leaves (UE-12) that enhanced the survival of infected nematodes without interfering with bacterial growth. In this study, we used a fluorescent transgenic reporter strain and observed that the repressed expression of the lys-7 defense gene in infected nematodes was restored in the presence of UE-12. Analysis of a selected panel of PMK-1 and DAF-16-regulated transcripts and loss-of-function mutants in these pathways indicates that the protective role of UE-12 is mediated via the p38 MAP kinase and insulin-like signaling pathways. Further analysis of a panel of known bioactive compounds of UE-12 proposed eupatorin (C18H16O7) as the possible candidate active molecule contributing to the anti-infective property of UE-12. Taken together, these findings strongly suggest that the O. stamineus leaf extract is a promising anti-infective agent that confers an advantage in survival against S. aureus infection by modulating the immune response of the infected host.

Original languageEnglish
Pages (from-to)644-655
Number of pages12
JournalBiology Open
Volume3
Issue number7
DOIs
Publication statusPublished - 15 Jul 2014

Fingerprint

Orthosiphon
Orthosiphon aristatus
immunomodulation
Immunomodulation
Caenorhabditis elegans
Anti-Infective Agents
Staphylococcus aureus
Molecules
p38 Mitogen-Activated Protein Kinases
anti-infective agents
Infection
Nematoda
infection
Screening
Genes
extracts
anti-infective properties
Insulin
Anti-Bacterial Agents
mitogen-activated protein kinase

Keywords

  • Anti-infective
  • C. elegans
  • Immunomodulation
  • O. stamineus
  • S. aureus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation. / Kong, Cin; Tan, Man Wah; Nathan, Sheila.

In: Biology Open, Vol. 3, No. 7, 15.07.2014, p. 644-655.

Research output: Contribution to journalArticle

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