Oro-pharyngeal carriage and antimicrobial susceptibility of streptococcus pneumoniae from healthy children

Noor Zetti Zainol Rashid, Ilina Isahak, Poh Jeann Chiam, Muhammad Hamdi Suki, Nur Ain Mohamad, Dalila Hussain, Tee Yee Sim, Mohd Hasni Ja`Afar, Yasmin Anum Mohd Yusof, Md. Mostafizur Rahman

Research output: Contribution to journalArticle

Abstract

Objectives: Streptococcus pneumoniae causes acute otitis media, pneumonia, meningitis and bacteraemia. This study aimed to determine the prevalence of Streptococcus pneumoniae oropharyngeal carriage in healthy children and the antimicrobial susceptibility in a daycare nursery and a government-managed orphanage in Kuala Lumpur during 2010.Methods: Throat swabs were obtained from 36 children of daycare nursery (open community) and from 84 orphans from orphanage (closed community) those did not receive any pneumococcal vaccine. Children were between births to 6 years of age. Antibiotic susceptibility of isolated strains was determined using disk diffusion method and Etest® (minimum inhibitory concentration). Results: Overall prevalence of Streptococcus pneumoniae of the children was 1.7% (2 out of 120). Prevalence of the bacteria in open community was 5.6% (2 out of 36) and no positive cases were recorded in orphanage (closed community, (p=0.161). Prevalence was 15.4% (2 out of 13) in children aged below 2 years in the open community. There was no association was found to exist between Streptococcus pneumoniae carriage with age (p=0.432) and gender (p=0.418). Serotyping showed serotype 11F for one isolate, while the other was non-typable. Both isolates were susceptible to penicillin, azithromycin, ceftriaxone and vancomycin. The serotype 11F isolate was susceptible while the non-typable isolate was resistant to erythromycin.Conclusions: The results demonstrated low prevalence of Streptococcus pneumoniae in healthy children. These findings may complement other studies to explore further risk factors for colonisation, antimicrobial susceptibility and serotype distribution of Streptococcus pneumoniae to help for the planning of immunization strategies.

Original languageEnglish
JournalInternet Journal of Microbiology
Volume11
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Streptococcus pneumoniae
Orphanages
Nurseries
Disk Diffusion Antimicrobial Tests
Serotyping
Pneumococcal Vaccines
Azithromycin
Ceftriaxone
Otitis Media
Microbial Sensitivity Tests
Erythromycin
Vancomycin
Bacteremia
Pharynx
Meningitis
Penicillins
Immunization
Pneumonia
Parturition
Anti-Bacterial Agents

Keywords

  • Antimicrobial susceptibility
  • Children
  • Colonisation
  • Oropharyngeal carriage
  • Serotype
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Infectious Diseases

Cite this

Oro-pharyngeal carriage and antimicrobial susceptibility of streptococcus pneumoniae from healthy children. / Zainol Rashid, Noor Zetti; Isahak, Ilina; Chiam, Poh Jeann; Suki, Muhammad Hamdi; Mohamad, Nur Ain; Hussain, Dalila; Sim, Tee Yee; Ja`Afar, Mohd Hasni; Mohd Yusof, Yasmin Anum; Rahman, Md. Mostafizur.

In: Internet Journal of Microbiology, Vol. 11, No. 1, 2013.

Research output: Contribution to journalArticle

Zainol Rashid, Noor Zetti ; Isahak, Ilina ; Chiam, Poh Jeann ; Suki, Muhammad Hamdi ; Mohamad, Nur Ain ; Hussain, Dalila ; Sim, Tee Yee ; Ja`Afar, Mohd Hasni ; Mohd Yusof, Yasmin Anum ; Rahman, Md. Mostafizur. / Oro-pharyngeal carriage and antimicrobial susceptibility of streptococcus pneumoniae from healthy children. In: Internet Journal of Microbiology. 2013 ; Vol. 11, No. 1.
@article{c18aba111e934a1e98523db2241dfc7a,
title = "Oro-pharyngeal carriage and antimicrobial susceptibility of streptococcus pneumoniae from healthy children",
abstract = "Objectives: Streptococcus pneumoniae causes acute otitis media, pneumonia, meningitis and bacteraemia. This study aimed to determine the prevalence of Streptococcus pneumoniae oropharyngeal carriage in healthy children and the antimicrobial susceptibility in a daycare nursery and a government-managed orphanage in Kuala Lumpur during 2010.Methods: Throat swabs were obtained from 36 children of daycare nursery (open community) and from 84 orphans from orphanage (closed community) those did not receive any pneumococcal vaccine. Children were between births to 6 years of age. Antibiotic susceptibility of isolated strains was determined using disk diffusion method and Etest{\circledR} (minimum inhibitory concentration). Results: Overall prevalence of Streptococcus pneumoniae of the children was 1.7{\%} (2 out of 120). Prevalence of the bacteria in open community was 5.6{\%} (2 out of 36) and no positive cases were recorded in orphanage (closed community, (p=0.161). Prevalence was 15.4{\%} (2 out of 13) in children aged below 2 years in the open community. There was no association was found to exist between Streptococcus pneumoniae carriage with age (p=0.432) and gender (p=0.418). Serotyping showed serotype 11F for one isolate, while the other was non-typable. Both isolates were susceptible to penicillin, azithromycin, ceftriaxone and vancomycin. The serotype 11F isolate was susceptible while the non-typable isolate was resistant to erythromycin.Conclusions: The results demonstrated low prevalence of Streptococcus pneumoniae in healthy children. These findings may complement other studies to explore further risk factors for colonisation, antimicrobial susceptibility and serotype distribution of Streptococcus pneumoniae to help for the planning of immunization strategies.",
keywords = "Antimicrobial susceptibility, Children, Colonisation, Oropharyngeal carriage, Serotype, Streptococcus pneumoniae",
author = "{Zainol Rashid}, {Noor Zetti} and Ilina Isahak and Chiam, {Poh Jeann} and Suki, {Muhammad Hamdi} and Mohamad, {Nur Ain} and Dalila Hussain and Sim, {Tee Yee} and Ja`Afar, {Mohd Hasni} and {Mohd Yusof}, {Yasmin Anum} and Rahman, {Md. Mostafizur}",
year = "2013",
doi = "10.5580/2cda",
language = "English",
volume = "11",
journal = "Internet Journal of Microbiology",
issn = "1937-8289",
publisher = "Internet Scientific Publications, LLC",
number = "1",

}

TY - JOUR

T1 - Oro-pharyngeal carriage and antimicrobial susceptibility of streptococcus pneumoniae from healthy children

AU - Zainol Rashid, Noor Zetti

AU - Isahak, Ilina

AU - Chiam, Poh Jeann

AU - Suki, Muhammad Hamdi

AU - Mohamad, Nur Ain

AU - Hussain, Dalila

AU - Sim, Tee Yee

AU - Ja`Afar, Mohd Hasni

AU - Mohd Yusof, Yasmin Anum

AU - Rahman, Md. Mostafizur

PY - 2013

Y1 - 2013

N2 - Objectives: Streptococcus pneumoniae causes acute otitis media, pneumonia, meningitis and bacteraemia. This study aimed to determine the prevalence of Streptococcus pneumoniae oropharyngeal carriage in healthy children and the antimicrobial susceptibility in a daycare nursery and a government-managed orphanage in Kuala Lumpur during 2010.Methods: Throat swabs were obtained from 36 children of daycare nursery (open community) and from 84 orphans from orphanage (closed community) those did not receive any pneumococcal vaccine. Children were between births to 6 years of age. Antibiotic susceptibility of isolated strains was determined using disk diffusion method and Etest® (minimum inhibitory concentration). Results: Overall prevalence of Streptococcus pneumoniae of the children was 1.7% (2 out of 120). Prevalence of the bacteria in open community was 5.6% (2 out of 36) and no positive cases were recorded in orphanage (closed community, (p=0.161). Prevalence was 15.4% (2 out of 13) in children aged below 2 years in the open community. There was no association was found to exist between Streptococcus pneumoniae carriage with age (p=0.432) and gender (p=0.418). Serotyping showed serotype 11F for one isolate, while the other was non-typable. Both isolates were susceptible to penicillin, azithromycin, ceftriaxone and vancomycin. The serotype 11F isolate was susceptible while the non-typable isolate was resistant to erythromycin.Conclusions: The results demonstrated low prevalence of Streptococcus pneumoniae in healthy children. These findings may complement other studies to explore further risk factors for colonisation, antimicrobial susceptibility and serotype distribution of Streptococcus pneumoniae to help for the planning of immunization strategies.

AB - Objectives: Streptococcus pneumoniae causes acute otitis media, pneumonia, meningitis and bacteraemia. This study aimed to determine the prevalence of Streptococcus pneumoniae oropharyngeal carriage in healthy children and the antimicrobial susceptibility in a daycare nursery and a government-managed orphanage in Kuala Lumpur during 2010.Methods: Throat swabs were obtained from 36 children of daycare nursery (open community) and from 84 orphans from orphanage (closed community) those did not receive any pneumococcal vaccine. Children were between births to 6 years of age. Antibiotic susceptibility of isolated strains was determined using disk diffusion method and Etest® (minimum inhibitory concentration). Results: Overall prevalence of Streptococcus pneumoniae of the children was 1.7% (2 out of 120). Prevalence of the bacteria in open community was 5.6% (2 out of 36) and no positive cases were recorded in orphanage (closed community, (p=0.161). Prevalence was 15.4% (2 out of 13) in children aged below 2 years in the open community. There was no association was found to exist between Streptococcus pneumoniae carriage with age (p=0.432) and gender (p=0.418). Serotyping showed serotype 11F for one isolate, while the other was non-typable. Both isolates were susceptible to penicillin, azithromycin, ceftriaxone and vancomycin. The serotype 11F isolate was susceptible while the non-typable isolate was resistant to erythromycin.Conclusions: The results demonstrated low prevalence of Streptococcus pneumoniae in healthy children. These findings may complement other studies to explore further risk factors for colonisation, antimicrobial susceptibility and serotype distribution of Streptococcus pneumoniae to help for the planning of immunization strategies.

KW - Antimicrobial susceptibility

KW - Children

KW - Colonisation

KW - Oropharyngeal carriage

KW - Serotype

KW - Streptococcus pneumoniae

UR - http://www.scopus.com/inward/record.url?scp=84874400405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874400405&partnerID=8YFLogxK

U2 - 10.5580/2cda

DO - 10.5580/2cda

M3 - Article

AN - SCOPUS:84874400405

VL - 11

JO - Internet Journal of Microbiology

JF - Internet Journal of Microbiology

SN - 1937-8289

IS - 1

ER -