Oral supplementation of L-glutathione prevents ultraviolet B-induced melanogenesis and oxidative stress in BALB/c mice

Tava Shelan Nagapan, Wenna Nallance Lim, Dayang Fredalina Basri, Ahmad Rohi Ghazali

Research output: Contribution to journalArticle

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Abstract

Dietary antioxidant supplements such as L-glutathione have gained considerable attention in dermatology and cosmeceutical fields. L-glutathione possesses antiaging, antimelanogenic, antioxidant, and anticancer properties. This study aimed to investigate the inhibitory effects of L-glutathione on melanogenesis activity and oxidative stress in ultraviolet B (UVB)-irradiated BALB/c mice. Eighteen female BALB/c mice were randomly divided into 3 groups: a control group (n=6), a group without UVB irradiation and L-glutathione administration; a UVB irradiated group (n=6), a group irradiated with a UVB dose of 250 mJ/cm2 for 3 min; and a treatment group (n=6), a group irradiated with UVB and treated with 100 mg/kg of L-glutathione by oral gavage. Treatment was given for 14 days, and UVB irradiation was given on days 9, 11, and 13. Oral L-glutathione significantly (P<0.05) reduced lipid peroxidation and elevated superoxide dismutase activity the and glutathione level. L-glutathione also inhibited melanin content and tyrosinase activity significantly (P<0.05) as compared with the UVB-irradiated group. Histopathological examination also showed that L-glutathione reduced the deposition of melanin pigment in the basal layer of the epidermis as compared with that in UVB-irradiated mice. All in all, the present study demonstrated that L-glutathione has the potential to be developed as a photoprotection agent against UVB-induced oxidative stress and melanogenesis.

Original languageEnglish
Pages (from-to)541-548
Number of pages8
JournalExperimental Animals
Volume68
Issue number4
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

melanogenesis
Oxidative stress
Glutathione
ultraviolet radiation
glutathione
mouth
Oxidative Stress
oxidative stress
mice
Melanins
melanin
Antioxidants
irradiation
Dermatology
Irradiation
antioxidants
Monophenol Monooxygenase
photostability
epidermis (animal)
Dietary Supplements

Keywords

  • L-glutathione
  • Melanogenesis
  • Oxidative stress
  • Photoprotection
  • Ultraviolet B (UVB)

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Oral supplementation of L-glutathione prevents ultraviolet B-induced melanogenesis and oxidative stress in BALB/c mice. / Nagapan, Tava Shelan; Lim, Wenna Nallance; Basri, Dayang Fredalina; Ghazali, Ahmad Rohi.

In: Experimental Animals, Vol. 68, No. 4, 01.01.2019, p. 541-548.

Research output: Contribution to journalArticle

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