Oral cancer secretome: Identification of cancer-associated proteins

Hong Yun Chang, Seen Yii Hor, Kue Peng Lim, Rosnah Binti Zain, Sok Ching Cheong, Mariati Abdul Rahman, Saiful Anuar Karsani

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study aims to identify cancer-associated proteins in the secretome of oral cancer cell lines. We have successfully established four primary cell cultures of normal cells with a limited lifespan without human telomerase reverse transcriptase (hTERT) immortalization. The secretome of these primary cell cultures were compared with that of oral cancer cell lines using 2DE. Thirty five protein spots were found to have changed in abundance. Unambiguous identification of these proteins was achieved by MALDI TOF/TOF. In silico analysis predicted that 24 of these proteins were secreted via classical or nonclassical mechanisms. The mRNA expression of six genes was found to correlate with the corresponding protein abundance. Ingenuity Pathway Analysis (IPA) core analysis revealed that the identified proteins were relevant in, and related to, cancer development with likely involvements in tumor growth, metastasis, hyperproliferation, tumorigenesis, neoplasia, hyperplasia, and cell transformation. In conclusion, we have demonstrated that a comparative study of the secretome of cancer versus normal cell lines can be used to identify cancer-associated proteins.

Original languageEnglish
Pages (from-to)2199-2208
Number of pages10
JournalElectrophoresis
Volume34
Issue number15
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Mouth Neoplasms
Neoplasms
Proteins
Primary Cell Culture
Cells
Cell culture
Cell Line
Core analysis
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Computer Simulation
Hyperplasia
Tumors
Carcinogenesis
Genes
Neoplasm Metastasis
Gene Expression
Messenger RNA
Growth

Keywords

  • Oral cancer
  • Proteomics
  • Secretome

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Chang, H. Y., Hor, S. Y., Lim, K. P., Zain, R. B., Cheong, S. C., Abdul Rahman, M., & Karsani, S. A. (2013). Oral cancer secretome: Identification of cancer-associated proteins. Electrophoresis, 34(15), 2199-2208. https://doi.org/10.1002/elps.201300126

Oral cancer secretome : Identification of cancer-associated proteins. / Chang, Hong Yun; Hor, Seen Yii; Lim, Kue Peng; Zain, Rosnah Binti; Cheong, Sok Ching; Abdul Rahman, Mariati; Karsani, Saiful Anuar.

In: Electrophoresis, Vol. 34, No. 15, 08.2013, p. 2199-2208.

Research output: Contribution to journalArticle

Chang, HY, Hor, SY, Lim, KP, Zain, RB, Cheong, SC, Abdul Rahman, M & Karsani, SA 2013, 'Oral cancer secretome: Identification of cancer-associated proteins', Electrophoresis, vol. 34, no. 15, pp. 2199-2208. https://doi.org/10.1002/elps.201300126
Chang, Hong Yun ; Hor, Seen Yii ; Lim, Kue Peng ; Zain, Rosnah Binti ; Cheong, Sok Ching ; Abdul Rahman, Mariati ; Karsani, Saiful Anuar. / Oral cancer secretome : Identification of cancer-associated proteins. In: Electrophoresis. 2013 ; Vol. 34, No. 15. pp. 2199-2208.
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