Oral administration of resveratrol ameliorates epidermal hyperplasia in ultraviolet B irradiated BALB/c mice

Tava Shelan Nagapan, Ahmad Rohi Ghazali, Dayang Fredalina Basri, Wenna Nallance Lim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Commercial sunscreens contain cosmetic preservatives which could exert phototoxic effect when exposed to sunlight. Natural sources, such as resveratrol, have gain attention in the cosmetic world as it has higher antioxidant capacity than vitamins C and E. Resveratrol also possesses strong anti-inflammatory, anti-cancer, and anti-aging properties. It also has proven to reduce epidermal hyperplasia and skin thickness. The aim of the study was to evaluate the ameliorative effect of resveratrol in reducing epidermal hyperplasia of mouse skin exposed to UVB irradiation. Eighteen female BALB/c mice were randomly divided into three groups: control group (n = 6), without Ultraviolet B irradiation and resveratrol administration; UVB exposure group (n = 6), irradiated with UVB dose of 250 mJ/cm2 for 3 minutes; and treatment group (n = 6), irradiated with UVB and treated with 0.02 ml of 200 mg/kg of resveratrol by oral gavage. Treatment was given for 14 days and UVB exposure was given on day 9, 11, and 13. On day 15, skin morphology was observed and skin-fold thickness was measured to evaluate edema. The mice were then sacrificed to obtain dorsal part of skin for histopathological observation using hematoxylin and eosin (H&E) stain. Resveratrol reduced skin scaling and erythema in UVB induced mice. Skin-fold thickness of resveratrol decreased significantly (p = 0.001) as compared to UVB irradiated group. H&E staining showed that resveratrol group reduced leukocyte infiltration and epidermal hyperplasia as compared to UVB exposure group. Hence, oral resveratrol was able to reduce skin thickness and epidermal hyperplasia and it has the potential to be developed as a natural alternative for photoprotection.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalJournal of Applied Pharmaceutical Science
Volume8
Issue number10
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Hyperplasia
Oral Administration
Skin
Cosmetics
resveratrol
Sunscreening Agents
Sunlight
Erythema
Hematoxylin
Eosine Yellowish-(YS)
Vitamin E
Ascorbic Acid
Edema
Leukocytes
Anti-Inflammatory Agents
Coloring Agents
Antioxidants
Observation
Staining and Labeling
Control Groups

Keywords

  • BALB/c mice
  • Resveratrol
  • Skin
  • Ultraviolet radiation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Oral administration of resveratrol ameliorates epidermal hyperplasia in ultraviolet B irradiated BALB/c mice. / Nagapan, Tava Shelan; Ghazali, Ahmad Rohi; Basri, Dayang Fredalina; Lim, Wenna Nallance.

In: Journal of Applied Pharmaceutical Science, Vol. 8, No. 10, 01.10.2018, p. 47-52.

Research output: Contribution to journalArticle

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abstract = "Commercial sunscreens contain cosmetic preservatives which could exert phototoxic effect when exposed to sunlight. Natural sources, such as resveratrol, have gain attention in the cosmetic world as it has higher antioxidant capacity than vitamins C and E. Resveratrol also possesses strong anti-inflammatory, anti-cancer, and anti-aging properties. It also has proven to reduce epidermal hyperplasia and skin thickness. The aim of the study was to evaluate the ameliorative effect of resveratrol in reducing epidermal hyperplasia of mouse skin exposed to UVB irradiation. Eighteen female BALB/c mice were randomly divided into three groups: control group (n = 6), without Ultraviolet B irradiation and resveratrol administration; UVB exposure group (n = 6), irradiated with UVB dose of 250 mJ/cm2 for 3 minutes; and treatment group (n = 6), irradiated with UVB and treated with 0.02 ml of 200 mg/kg of resveratrol by oral gavage. Treatment was given for 14 days and UVB exposure was given on day 9, 11, and 13. On day 15, skin morphology was observed and skin-fold thickness was measured to evaluate edema. The mice were then sacrificed to obtain dorsal part of skin for histopathological observation using hematoxylin and eosin (H&E) stain. Resveratrol reduced skin scaling and erythema in UVB induced mice. Skin-fold thickness of resveratrol decreased significantly (p = 0.001) as compared to UVB irradiated group. H&E staining showed that resveratrol group reduced leukocyte infiltration and epidermal hyperplasia as compared to UVB exposure group. Hence, oral resveratrol was able to reduce skin thickness and epidermal hyperplasia and it has the potential to be developed as a natural alternative for photoprotection.",
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