Optimizing of Trichoderma viride cultivation in submerged state fermentation

Hayyan Ismaeil Al-Taweil, Mohammad Bin Osman, Aidil Abdul Hamid, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Problems statement: A study in Malaysia had shown a strain of Trichoderma viride was isolated from the soil. Questions were raised whether Trichoderma viride submerged state fermentation affected by parameters and which of them is effective? Approach: To investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80), glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0) rpm. Experiments were performed in triplicate and the results were statistically analyzed using computer software Response Surface Methodology (RSM) using a Box-Behnken design was applied to batch cultures of T. viride, for identifying the effects of process variables (carbon sources, nitrogen sources, temperature, RPM and PH). The fermentation was carried out in shake flasks using a complex medium fungal biomass the mycelium was filtered through filter paper (Whatman No. 40). It was washed first with distilled water tow times. The washed mycelium was dried at 105-1°C to constant mass. It was placed in the desiccators and then the mass was determined. Results: The fermentation pattern was studied to investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80) glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0) rpm; for Trichoderma biomass production for biotechnological uses (biocontrol agent). Optimum parameters and maximum biomass production were studied. The maximum biomass production of 13.6 g mL-1 mycelium was noted after 5 days. Although maximum fungal biomass presented maximum growth rate that observed between the 3rd and 4th days of fermentation. At 3rd day 13.2 g L-1 fungal dry mass was present, after that there was a slight decrease in the mycelial dry mass. A Box-Behnken experimental design was used to investigate the effects of five factors; concentrations of carbon g L-1; (10, 45 and 80) glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0), rpm; (100, 175 and 250) on the concentrations of biomass produced in batch cultures of Trichoderma viride. Optimal medium for maximizing the production of biomass in batch cultures of T. viride should contain 45 g L-1 C, 0.35 g L-1 N, 30 Temp, 175 rpm and Ph 6 for 5 days fermentation. Optimization of Trichoderma cultivation in submerged state fermentation to produce the optimum biomass as stage of biocontrol agent and biofertilizer production which made the production line more significant. Conclusion: Based on a statistically designed search, results indicated that an optimal medium for maximizing the production of biomass in batch cultures of T. viride should contain 45 g L-1 C, 0.35 g L-1 N, 30 Temp, 175 rpm and Ph 6. This composition can yield the optimum biomass 5 days of culture. The identified optimal medium is rich in carbon but provided a limiting level of nitrogen.

Original languageEnglish
Pages (from-to)1284-1288
Number of pages5
JournalAmerican Journal of Applied Sciences
Volume6
Issue number7
DOIs
Publication statusPublished - 2009

Fingerprint

Trichoderma viride
fermentation
biomass production
ammonium sulfate
carbon
biomass
mycelium
nitrogen
Trichoderma
glucose
biological control agents
temperature
biofertilizers
response surface methodology
Malaysia
experimental design

Keywords

  • Biomass
  • Fermentation
  • Submerged state
  • Trichoderma

ASJC Scopus subject areas

  • General

Cite this

Optimizing of Trichoderma viride cultivation in submerged state fermentation. / Al-Taweil, Hayyan Ismaeil; Osman, Mohammad Bin; Abdul Hamid, Aidil; Wan Yusoff, Wan Mohtar.

In: American Journal of Applied Sciences, Vol. 6, No. 7, 2009, p. 1284-1288.

Research output: Contribution to journalArticle

Al-Taweil, Hayyan Ismaeil ; Osman, Mohammad Bin ; Abdul Hamid, Aidil ; Wan Yusoff, Wan Mohtar. / Optimizing of Trichoderma viride cultivation in submerged state fermentation. In: American Journal of Applied Sciences. 2009 ; Vol. 6, No. 7. pp. 1284-1288.
@article{213775b116d34826a89e0b67ab308736,
title = "Optimizing of Trichoderma viride cultivation in submerged state fermentation",
abstract = "Problems statement: A study in Malaysia had shown a strain of Trichoderma viride was isolated from the soil. Questions were raised whether Trichoderma viride submerged state fermentation affected by parameters and which of them is effective? Approach: To investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80), glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0) rpm. Experiments were performed in triplicate and the results were statistically analyzed using computer software Response Surface Methodology (RSM) using a Box-Behnken design was applied to batch cultures of T. viride, for identifying the effects of process variables (carbon sources, nitrogen sources, temperature, RPM and PH). The fermentation was carried out in shake flasks using a complex medium fungal biomass the mycelium was filtered through filter paper (Whatman No. 40). It was washed first with distilled water tow times. The washed mycelium was dried at 105-1°C to constant mass. It was placed in the desiccators and then the mass was determined. Results: The fermentation pattern was studied to investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80) glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0) rpm; for Trichoderma biomass production for biotechnological uses (biocontrol agent). Optimum parameters and maximum biomass production were studied. The maximum biomass production of 13.6 g mL-1 mycelium was noted after 5 days. Although maximum fungal biomass presented maximum growth rate that observed between the 3rd and 4th days of fermentation. At 3rd day 13.2 g L-1 fungal dry mass was present, after that there was a slight decrease in the mycelial dry mass. A Box-Behnken experimental design was used to investigate the effects of five factors; concentrations of carbon g L-1; (10, 45 and 80) glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0), rpm; (100, 175 and 250) on the concentrations of biomass produced in batch cultures of Trichoderma viride. Optimal medium for maximizing the production of biomass in batch cultures of T. viride should contain 45 g L-1 C, 0.35 g L-1 N, 30 Temp, 175 rpm and Ph 6 for 5 days fermentation. Optimization of Trichoderma cultivation in submerged state fermentation to produce the optimum biomass as stage of biocontrol agent and biofertilizer production which made the production line more significant. Conclusion: Based on a statistically designed search, results indicated that an optimal medium for maximizing the production of biomass in batch cultures of T. viride should contain 45 g L-1 C, 0.35 g L-1 N, 30 Temp, 175 rpm and Ph 6. This composition can yield the optimum biomass 5 days of culture. The identified optimal medium is rich in carbon but provided a limiting level of nitrogen.",
keywords = "Biomass, Fermentation, Submerged state, Trichoderma",
author = "Al-Taweil, {Hayyan Ismaeil} and Osman, {Mohammad Bin} and {Abdul Hamid}, Aidil and {Wan Yusoff}, {Wan Mohtar}",
year = "2009",
doi = "10.3844/ajassp.2009.1284.1288",
language = "English",
volume = "6",
pages = "1284--1288",
journal = "American Journal of Applied Sciences",
issn = "1546-9239",
publisher = "Science Publications",
number = "7",

}

TY - JOUR

T1 - Optimizing of Trichoderma viride cultivation in submerged state fermentation

AU - Al-Taweil, Hayyan Ismaeil

AU - Osman, Mohammad Bin

AU - Abdul Hamid, Aidil

AU - Wan Yusoff, Wan Mohtar

PY - 2009

Y1 - 2009

N2 - Problems statement: A study in Malaysia had shown a strain of Trichoderma viride was isolated from the soil. Questions were raised whether Trichoderma viride submerged state fermentation affected by parameters and which of them is effective? Approach: To investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80), glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0) rpm. Experiments were performed in triplicate and the results were statistically analyzed using computer software Response Surface Methodology (RSM) using a Box-Behnken design was applied to batch cultures of T. viride, for identifying the effects of process variables (carbon sources, nitrogen sources, temperature, RPM and PH). The fermentation was carried out in shake flasks using a complex medium fungal biomass the mycelium was filtered through filter paper (Whatman No. 40). It was washed first with distilled water tow times. The washed mycelium was dried at 105-1°C to constant mass. It was placed in the desiccators and then the mass was determined. Results: The fermentation pattern was studied to investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80) glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0) rpm; for Trichoderma biomass production for biotechnological uses (biocontrol agent). Optimum parameters and maximum biomass production were studied. The maximum biomass production of 13.6 g mL-1 mycelium was noted after 5 days. Although maximum fungal biomass presented maximum growth rate that observed between the 3rd and 4th days of fermentation. At 3rd day 13.2 g L-1 fungal dry mass was present, after that there was a slight decrease in the mycelial dry mass. A Box-Behnken experimental design was used to investigate the effects of five factors; concentrations of carbon g L-1; (10, 45 and 80) glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0), rpm; (100, 175 and 250) on the concentrations of biomass produced in batch cultures of Trichoderma viride. Optimal medium for maximizing the production of biomass in batch cultures of T. viride should contain 45 g L-1 C, 0.35 g L-1 N, 30 Temp, 175 rpm and Ph 6 for 5 days fermentation. Optimization of Trichoderma cultivation in submerged state fermentation to produce the optimum biomass as stage of biocontrol agent and biofertilizer production which made the production line more significant. Conclusion: Based on a statistically designed search, results indicated that an optimal medium for maximizing the production of biomass in batch cultures of T. viride should contain 45 g L-1 C, 0.35 g L-1 N, 30 Temp, 175 rpm and Ph 6. This composition can yield the optimum biomass 5 days of culture. The identified optimal medium is rich in carbon but provided a limiting level of nitrogen.

AB - Problems statement: A study in Malaysia had shown a strain of Trichoderma viride was isolated from the soil. Questions were raised whether Trichoderma viride submerged state fermentation affected by parameters and which of them is effective? Approach: To investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80), glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0) rpm. Experiments were performed in triplicate and the results were statistically analyzed using computer software Response Surface Methodology (RSM) using a Box-Behnken design was applied to batch cultures of T. viride, for identifying the effects of process variables (carbon sources, nitrogen sources, temperature, RPM and PH). The fermentation was carried out in shake flasks using a complex medium fungal biomass the mycelium was filtered through filter paper (Whatman No. 40). It was washed first with distilled water tow times. The washed mycelium was dried at 105-1°C to constant mass. It was placed in the desiccators and then the mass was determined. Results: The fermentation pattern was studied to investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80) glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0) rpm; for Trichoderma biomass production for biotechnological uses (biocontrol agent). Optimum parameters and maximum biomass production were studied. The maximum biomass production of 13.6 g mL-1 mycelium was noted after 5 days. Although maximum fungal biomass presented maximum growth rate that observed between the 3rd and 4th days of fermentation. At 3rd day 13.2 g L-1 fungal dry mass was present, after that there was a slight decrease in the mycelial dry mass. A Box-Behnken experimental design was used to investigate the effects of five factors; concentrations of carbon g L-1; (10, 45 and 80) glucose, nitrogen g L-1; (0.10, 0.35 and 0.60) ammonium sulfate, temperature; (20, 30 and 40), PH; (4.0, 6.0 and 8.0), rpm; (100, 175 and 250) on the concentrations of biomass produced in batch cultures of Trichoderma viride. Optimal medium for maximizing the production of biomass in batch cultures of T. viride should contain 45 g L-1 C, 0.35 g L-1 N, 30 Temp, 175 rpm and Ph 6 for 5 days fermentation. Optimization of Trichoderma cultivation in submerged state fermentation to produce the optimum biomass as stage of biocontrol agent and biofertilizer production which made the production line more significant. Conclusion: Based on a statistically designed search, results indicated that an optimal medium for maximizing the production of biomass in batch cultures of T. viride should contain 45 g L-1 C, 0.35 g L-1 N, 30 Temp, 175 rpm and Ph 6. This composition can yield the optimum biomass 5 days of culture. The identified optimal medium is rich in carbon but provided a limiting level of nitrogen.

KW - Biomass

KW - Fermentation

KW - Submerged state

KW - Trichoderma

UR - http://www.scopus.com/inward/record.url?scp=70349283480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349283480&partnerID=8YFLogxK

U2 - 10.3844/ajassp.2009.1284.1288

DO - 10.3844/ajassp.2009.1284.1288

M3 - Article

AN - SCOPUS:70349283480

VL - 6

SP - 1284

EP - 1288

JO - American Journal of Applied Sciences

JF - American Journal of Applied Sciences

SN - 1546-9239

IS - 7

ER -