Optimized reuse and bioconversion from retentate of pre-filtered palm oil mill effluent (POME) into microbial protease by Aspergillus terreus using response surface methodology

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: The membrane filtration process enables the treatment of wastewater, producing permeate which is less polluted. However, disposal is usually required for the retentate, which is produced as a concentrated constituent along with the permeate. In this study, the authors explored the possibility of reusing, rather than disposing of, the retentate of pre-filtered palm oil mill effluent (POME) as a fermentation substrate in protease production by a wild type strain of Aspergillus terreus IMI 282743. In addition, the quantitative and interactive effects of the concentration factor for retentate, temperature, inoculum concentration, and fermentation time on the optimization of protease production were investigated using response surface methodology (RSM). Results: Using RSM, the optimum conditions were found to be a concentration factor of 7.27, temperature of 37.95 °C, inoculum concentration of 1.30% (v/v) and fermentation time of 3.83 days. The protease production was increased 4.37-fold in comparison with the results obtained under non-optimized conditions. Conclusion: To a certain extent, protease production could be enhanced with an increase in concentration factor and temperature, and a decrease of inoculum concentration and fermentation time. Also, POME retentate was found to be a good substrate for protease production with high product activity and without nutrient supplementation.

Original languageEnglish
Pages (from-to)1390-1396
Number of pages7
JournalJournal of Chemical Technology and Biotechnology
Volume84
Issue number9
DOIs
Publication statusPublished - 2009

Fingerprint

Bioconversion
Palm oil
Aspergillus
fermentation
Effluents
mill
Peptide Hydrolases
Fermentation
effluent
oil
Temperature
substrate
temperature
Substrates
Waste Water
Nutrients
membrane
fold
Wastewater
wastewater

Keywords

  • Aspergillus terreus
  • Palm oil mill effluent (POME)
  • Protease
  • Response surface methodology (RSM)
  • Retentate
  • Ultrafiltration

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Renewable Energy, Sustainability and the Environment
  • Biotechnology
  • Chemical Engineering(all)
  • Fuel Technology

Cite this

@article{dad913fae5734341a6760b803f3d3a7b,
title = "Optimized reuse and bioconversion from retentate of pre-filtered palm oil mill effluent (POME) into microbial protease by Aspergillus terreus using response surface methodology",
abstract = "Background: The membrane filtration process enables the treatment of wastewater, producing permeate which is less polluted. However, disposal is usually required for the retentate, which is produced as a concentrated constituent along with the permeate. In this study, the authors explored the possibility of reusing, rather than disposing of, the retentate of pre-filtered palm oil mill effluent (POME) as a fermentation substrate in protease production by a wild type strain of Aspergillus terreus IMI 282743. In addition, the quantitative and interactive effects of the concentration factor for retentate, temperature, inoculum concentration, and fermentation time on the optimization of protease production were investigated using response surface methodology (RSM). Results: Using RSM, the optimum conditions were found to be a concentration factor of 7.27, temperature of 37.95 °C, inoculum concentration of 1.30{\%} (v/v) and fermentation time of 3.83 days. The protease production was increased 4.37-fold in comparison with the results obtained under non-optimized conditions. Conclusion: To a certain extent, protease production could be enhanced with an increase in concentration factor and temperature, and a decrease of inoculum concentration and fermentation time. Also, POME retentate was found to be a good substrate for protease production with high product activity and without nutrient supplementation.",
keywords = "Aspergillus terreus, Palm oil mill effluent (POME), Protease, Response surface methodology (RSM), Retentate, Ultrafiltration",
author = "Wu, {Ta Yeong} and Mohammad, {Abdul Wahab} and {Md Jahim}, Jamaliah and Nurina Anuar",
year = "2009",
doi = "10.1002/jctb.2194",
language = "English",
volume = "84",
pages = "1390--1396",
journal = "Journal of Chemical Technology and Biotechnology",
issn = "0268-2575",
publisher = "John Wiley and Sons Ltd",
number = "9",

}

TY - JOUR

T1 - Optimized reuse and bioconversion from retentate of pre-filtered palm oil mill effluent (POME) into microbial protease by Aspergillus terreus using response surface methodology

AU - Wu, Ta Yeong

AU - Mohammad, Abdul Wahab

AU - Md Jahim, Jamaliah

AU - Anuar, Nurina

PY - 2009

Y1 - 2009

N2 - Background: The membrane filtration process enables the treatment of wastewater, producing permeate which is less polluted. However, disposal is usually required for the retentate, which is produced as a concentrated constituent along with the permeate. In this study, the authors explored the possibility of reusing, rather than disposing of, the retentate of pre-filtered palm oil mill effluent (POME) as a fermentation substrate in protease production by a wild type strain of Aspergillus terreus IMI 282743. In addition, the quantitative and interactive effects of the concentration factor for retentate, temperature, inoculum concentration, and fermentation time on the optimization of protease production were investigated using response surface methodology (RSM). Results: Using RSM, the optimum conditions were found to be a concentration factor of 7.27, temperature of 37.95 °C, inoculum concentration of 1.30% (v/v) and fermentation time of 3.83 days. The protease production was increased 4.37-fold in comparison with the results obtained under non-optimized conditions. Conclusion: To a certain extent, protease production could be enhanced with an increase in concentration factor and temperature, and a decrease of inoculum concentration and fermentation time. Also, POME retentate was found to be a good substrate for protease production with high product activity and without nutrient supplementation.

AB - Background: The membrane filtration process enables the treatment of wastewater, producing permeate which is less polluted. However, disposal is usually required for the retentate, which is produced as a concentrated constituent along with the permeate. In this study, the authors explored the possibility of reusing, rather than disposing of, the retentate of pre-filtered palm oil mill effluent (POME) as a fermentation substrate in protease production by a wild type strain of Aspergillus terreus IMI 282743. In addition, the quantitative and interactive effects of the concentration factor for retentate, temperature, inoculum concentration, and fermentation time on the optimization of protease production were investigated using response surface methodology (RSM). Results: Using RSM, the optimum conditions were found to be a concentration factor of 7.27, temperature of 37.95 °C, inoculum concentration of 1.30% (v/v) and fermentation time of 3.83 days. The protease production was increased 4.37-fold in comparison with the results obtained under non-optimized conditions. Conclusion: To a certain extent, protease production could be enhanced with an increase in concentration factor and temperature, and a decrease of inoculum concentration and fermentation time. Also, POME retentate was found to be a good substrate for protease production with high product activity and without nutrient supplementation.

KW - Aspergillus terreus

KW - Palm oil mill effluent (POME)

KW - Protease

KW - Response surface methodology (RSM)

KW - Retentate

KW - Ultrafiltration

UR - http://www.scopus.com/inward/record.url?scp=70349502113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349502113&partnerID=8YFLogxK

U2 - 10.1002/jctb.2194

DO - 10.1002/jctb.2194

M3 - Article

AN - SCOPUS:70349502113

VL - 84

SP - 1390

EP - 1396

JO - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 9

ER -