Optimized growth of Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM)

Jacqueline Xiao Wen Hay, Ta Yeong Wu, Chee Yang Teh, Jamaliah Md Jahim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This study presents optimization of growth parameters (temperature, pH, ammonium conc. and inoculum size) to achieve an economical viable biohydrogen production process by Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM) followed by analysis of light and agitation effects on bacterial growth using optimized conditions. Optimized growth conditions on third day of fermentation, when R. sphaeroides O.U.001 was cultured aerobically under the presence of light (510 lux), were found as follows: temperature, 30.3°C; pH, 4.71; ammonium conc., 0.60 g/l; and inoculum size, 6.02% (v/v). Light did not give a significant effect on overall growth of R. sphaeroides O.U.001. Agitation greatly influenced bacterial growth as a result of higher dissolved oxygen and dispersion of macromolecules in shake culture.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalJournal of Scientific and Industrial Research
Volume71
Issue number2
Publication statusPublished - Feb 2012

Fingerprint

Rhodobacter sphaeroides
response surface methodology
agitation
microbial growth
inoculum
hydrogen production
dissolved oxygen
temperature
fermentation

Keywords

  • Biohydrogen
  • Photofermentation
  • Response surface methodology (rsm)
  • Rhodobacter sphaeroides o.u.001

ASJC Scopus subject areas

  • General

Cite this

Optimized growth of Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM). / Hay, Jacqueline Xiao Wen; Wu, Ta Yeong; Teh, Chee Yang; Md Jahim, Jamaliah.

In: Journal of Scientific and Industrial Research, Vol. 71, No. 2, 02.2012, p. 149-154.

Research output: Contribution to journalArticle

@article{0d885ae298584956b4b243b25072e36c,
title = "Optimized growth of Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM)",
abstract = "This study presents optimization of growth parameters (temperature, pH, ammonium conc. and inoculum size) to achieve an economical viable biohydrogen production process by Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM) followed by analysis of light and agitation effects on bacterial growth using optimized conditions. Optimized growth conditions on third day of fermentation, when R. sphaeroides O.U.001 was cultured aerobically under the presence of light (510 lux), were found as follows: temperature, 30.3°C; pH, 4.71; ammonium conc., 0.60 g/l; and inoculum size, 6.02{\%} (v/v). Light did not give a significant effect on overall growth of R. sphaeroides O.U.001. Agitation greatly influenced bacterial growth as a result of higher dissolved oxygen and dispersion of macromolecules in shake culture.",
keywords = "Biohydrogen, Photofermentation, Response surface methodology (rsm), Rhodobacter sphaeroides o.u.001",
author = "Hay, {Jacqueline Xiao Wen} and Wu, {Ta Yeong} and Teh, {Chee Yang} and {Md Jahim}, Jamaliah",
year = "2012",
month = "2",
language = "English",
volume = "71",
pages = "149--154",
journal = "Journal of Scientific and Industrial Research",
issn = "0022-4456",
publisher = "National Institute of Science Communication and Information Resources (NISCAIR)",
number = "2",

}

TY - JOUR

T1 - Optimized growth of Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM)

AU - Hay, Jacqueline Xiao Wen

AU - Wu, Ta Yeong

AU - Teh, Chee Yang

AU - Md Jahim, Jamaliah

PY - 2012/2

Y1 - 2012/2

N2 - This study presents optimization of growth parameters (temperature, pH, ammonium conc. and inoculum size) to achieve an economical viable biohydrogen production process by Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM) followed by analysis of light and agitation effects on bacterial growth using optimized conditions. Optimized growth conditions on third day of fermentation, when R. sphaeroides O.U.001 was cultured aerobically under the presence of light (510 lux), were found as follows: temperature, 30.3°C; pH, 4.71; ammonium conc., 0.60 g/l; and inoculum size, 6.02% (v/v). Light did not give a significant effect on overall growth of R. sphaeroides O.U.001. Agitation greatly influenced bacterial growth as a result of higher dissolved oxygen and dispersion of macromolecules in shake culture.

AB - This study presents optimization of growth parameters (temperature, pH, ammonium conc. and inoculum size) to achieve an economical viable biohydrogen production process by Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM) followed by analysis of light and agitation effects on bacterial growth using optimized conditions. Optimized growth conditions on third day of fermentation, when R. sphaeroides O.U.001 was cultured aerobically under the presence of light (510 lux), were found as follows: temperature, 30.3°C; pH, 4.71; ammonium conc., 0.60 g/l; and inoculum size, 6.02% (v/v). Light did not give a significant effect on overall growth of R. sphaeroides O.U.001. Agitation greatly influenced bacterial growth as a result of higher dissolved oxygen and dispersion of macromolecules in shake culture.

KW - Biohydrogen

KW - Photofermentation

KW - Response surface methodology (rsm)

KW - Rhodobacter sphaeroides o.u.001

UR - http://www.scopus.com/inward/record.url?scp=84856693572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856693572&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84856693572

VL - 71

SP - 149

EP - 154

JO - Journal of Scientific and Industrial Research

JF - Journal of Scientific and Industrial Research

SN - 0022-4456

IS - 2

ER -