Optimization of vanillin production using isoeugenol as substrate by Aspergillus niger I-1472

M. C. Tan, S. L. Liew, Mohamad Yusof Maskat, Wan Aida Wan Mustapha, Osman Hassan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Response Surface Methodology (RSM) was used in the study to optimize the production of vanillin from isoeugenol through fermentation by Aspergillus niger I-1472. Three factors were studied which include amount of isoeugenol, resin (Amberlite XAD-4) and Span 80. During fermentation, isoeugenol as substrate were vortexed with Span 80 and added into the culture on Day 4. Resin (Amberlite XAD-4) was added into the medium the following day. The predicted optimum medium combination consisted of 3.61 g/L of isoeugenol, 5.8% (g/mL) of Amberlite XAD-4 resin and 0.37% of Span 80 with an expected vanillin production of 0.137 g/L. Verification test showed that the model produced similar predicted and experimental values.

Original languageEnglish
Pages (from-to)1651-1656
Number of pages6
JournalInternational Food Research Journal
Volume22
Issue number4
Publication statusPublished - 2015

Fingerprint

isoeugenol
Aspergillus niger
vanillin
resins
fermentation
Fermentation
response surface methodology
XAD-4 resin
testing

Keywords

  • Aspergillus niger I-1472
  • Biotransformation
  • Isoeugenol
  • Response surface methodology
  • Vanillin

ASJC Scopus subject areas

  • Food Science

Cite this

Optimization of vanillin production using isoeugenol as substrate by Aspergillus niger I-1472. / Tan, M. C.; Liew, S. L.; Maskat, Mohamad Yusof; Wan Mustapha, Wan Aida; Hassan, Osman.

In: International Food Research Journal, Vol. 22, No. 4, 2015, p. 1651-1656.

Research output: Contribution to journalArticle

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AU - Hassan, Osman

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