Optimization of the process conditions for cyclodextrin glucanotransferase production using response surface methodology

Hanan Moawia Ibrahim, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid, Rosli M D Illias, Othman Omar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Statistical experimental design was used to optimize the physical conditions such as temperature and speed of agitation in shake flask culture to enhance cyclodextrin glucanotransferase (CGTase) production from Bacillus sp. G1. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular CGTase was achieved (41.4 U/ml) when the speed of agitation and temperature were 147.7 rpm, 34.48°C respectively.

Original languageEnglish
Pages (from-to)297-300
Number of pages4
JournalAsian Journal of Microbiology, Biotechnology and Environmental Sciences
Volume5
Issue number3
Publication statusPublished - 2003

Fingerprint

experimental design
Research Design
Batch Cell Culture Techniques
Temperature
Bacillus
temperature
cyclomaltodextrin glucanotransferase
response surface methodology
speed
physical conditions

Keywords

  • Cyclodextrin glucanotransferase
  • Response surface methodology

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

@article{6ee93c32f5764b9fa6c41cf1f43c2be0,
title = "Optimization of the process conditions for cyclodextrin glucanotransferase production using response surface methodology",
abstract = "Statistical experimental design was used to optimize the physical conditions such as temperature and speed of agitation in shake flask culture to enhance cyclodextrin glucanotransferase (CGTase) production from Bacillus sp. G1. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular CGTase was achieved (41.4 U/ml) when the speed of agitation and temperature were 147.7 rpm, 34.48°C respectively.",
keywords = "Cyclodextrin glucanotransferase, Response surface methodology",
author = "Ibrahim, {Hanan Moawia} and {Wan Yusoff}, {Wan Mohtar} and {Abdul Hamid}, Aidil and Illias, {Rosli M D} and Othman Omar",
year = "2003",
language = "English",
volume = "5",
pages = "297--300",
journal = "Asian Journal of Microbiology, Biotechnology and Environmental Sciences",
issn = "0972-3005",
publisher = "Global Publications",
number = "3",

}

TY - JOUR

T1 - Optimization of the process conditions for cyclodextrin glucanotransferase production using response surface methodology

AU - Ibrahim, Hanan Moawia

AU - Wan Yusoff, Wan Mohtar

AU - Abdul Hamid, Aidil

AU - Illias, Rosli M D

AU - Omar, Othman

PY - 2003

Y1 - 2003

N2 - Statistical experimental design was used to optimize the physical conditions such as temperature and speed of agitation in shake flask culture to enhance cyclodextrin glucanotransferase (CGTase) production from Bacillus sp. G1. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular CGTase was achieved (41.4 U/ml) when the speed of agitation and temperature were 147.7 rpm, 34.48°C respectively.

AB - Statistical experimental design was used to optimize the physical conditions such as temperature and speed of agitation in shake flask culture to enhance cyclodextrin glucanotransferase (CGTase) production from Bacillus sp. G1. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular CGTase was achieved (41.4 U/ml) when the speed of agitation and temperature were 147.7 rpm, 34.48°C respectively.

KW - Cyclodextrin glucanotransferase

KW - Response surface methodology

UR - http://www.scopus.com/inward/record.url?scp=0346401567&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346401567&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0346401567

VL - 5

SP - 297

EP - 300

JO - Asian Journal of Microbiology, Biotechnology and Environmental Sciences

JF - Asian Journal of Microbiology, Biotechnology and Environmental Sciences

SN - 0972-3005

IS - 3

ER -